Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 4305356..4305942 Replicon chromosome
Accession NZ_CP077806
Organism Klebsiella pneumoniae strain FF979

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag KU665_RS20455 Protein ID WP_002920800.1
Coordinates 4305574..4305942 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag KU665_RS20450 Protein ID WP_004174006.1
Coordinates 4305356..4305577 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU665_RS20430 (KU665_20390) 4301513..4302439 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KU665_RS20435 (KU665_20395) 4302436..4303713 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
KU665_RS20440 (KU665_20400) 4303710..4304477 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KU665_RS20445 (KU665_20405) 4304479..4305192 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
KU665_RS20450 (KU665_20410) 4305356..4305577 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KU665_RS20455 (KU665_20415) 4305574..4305942 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KU665_RS20460 (KU665_20420) 4306294..4307610 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
KU665_RS20465 (KU665_20425) 4307717..4308604 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
KU665_RS20470 (KU665_20430) 4308601..4309446 + 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
KU665_RS20475 (KU665_20435) 4309448..4310518 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4265224..4311255 46031


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T207868 WP_002920800.1 NZ_CP077806:4305574-4305942 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T207868 NZ_CP103529:2827495-2827602 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT207868 WP_004174006.1 NZ_CP077806:4305356-4305577 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT207868 NZ_CP103529:c2827448-2827382 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References