Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 3316577..3317387 Replicon chromosome
Accession NZ_CP077806
Organism Klebsiella pneumoniae strain FF979

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KU665_RS15815 Protein ID WP_004178461.1
Coordinates 3316577..3317110 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KU665_RS15820 Protein ID WP_002887278.1
Coordinates 3317121..3317387 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU665_RS15810 (KU665_15785) 3315408..3316529 + 1122 WP_002887282.1 cupin domain-containing protein -
KU665_RS15815 (KU665_15790) 3316577..3317110 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KU665_RS15820 (KU665_15795) 3317121..3317387 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KU665_RS15825 (KU665_15800) 3317490..3318923 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
KU665_RS15830 (KU665_15805) 3318913..3319596 - 684 WP_251335724.1 copper/silver response regulator transcription factor -
KU665_RS15835 (KU665_15810) 3319768..3321153 + 1386 WP_228938596.1 efflux transporter outer membrane subunit -
KU665_RS15840 (KU665_15815) 3321171..3321515 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T207864 WP_004178461.1 NZ_CP077806:c3317110-3316577 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T207864 NZ_CP103529:2214367-2214470 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT207864 WP_002887278.1 NZ_CP077806:c3317387-3317121 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT207864 NZ_CP103529:c2214508-2214237 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References