Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2699499..2700118 Replicon chromosome
Accession NZ_CP077806
Organism Klebsiella pneumoniae strain FF979

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KU665_RS12885 Protein ID WP_002892050.1
Coordinates 2699900..2700118 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KU665_RS12880 Protein ID WP_002892066.1
Coordinates 2699499..2699873 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU665_RS12870 (KU665_12850) 2694651..2695844 + 1194 WP_251335657.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KU665_RS12875 (KU665_12855) 2695867..2699013 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KU665_RS12880 (KU665_12860) 2699499..2699873 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KU665_RS12885 (KU665_12865) 2699900..2700118 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KU665_RS12890 (KU665_12870) 2700277..2700843 + 567 WP_002892030.1 maltose O-acetyltransferase -
KU665_RS12895 (KU665_12875) 2700815..2700955 - 141 WP_004147370.1 hypothetical protein -
KU665_RS12900 (KU665_12880) 2700976..2701446 + 471 WP_002892026.1 YlaC family protein -
KU665_RS12905 (KU665_12885) 2701421..2702872 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KU665_RS12910 (KU665_12890) 2702973..2703671 + 699 WP_002892021.1 GNAT family protein -
KU665_RS12915 (KU665_12895) 2703668..2703808 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KU665_RS12920 (KU665_12900) 2703808..2704071 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T207863 WP_002892050.1 NZ_CP077806:2699900-2700118 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T207863 NZ_CP103529:c1947885-1947508 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCCCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT207863 WP_002892066.1 NZ_CP077806:2699499-2699873 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT207863 NZ_CP103529:c1948343-1947975 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References