Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2513272..2513862 Replicon chromosome
Accession NZ_CP077805
Organism Klebsiella variicola strain FF907

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag KU664_RS12175 Protein ID WP_008804165.1
Coordinates 2513530..2513862 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag KU664_RS12170 Protein ID WP_012541132.1
Coordinates 2513272..2513529 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU664_RS12150 (KU664_12115) 2508882..2509457 + 576 WP_012541129.1 hypothetical protein -
KU664_RS12155 (KU664_12120) 2509636..2510472 + 837 WP_008804155.1 alpha/beta hydrolase -
KU664_RS12160 (KU664_12125) 2510678..2511649 + 972 WP_072124611.1 sensor domain-containing diguanylate cyclase -
KU664_RS12165 (KU664_12130) 2511646..2512745 - 1100 Protein_2362 AarF/UbiB family protein -
KU664_RS12170 (KU664_12135) 2513272..2513529 + 258 WP_012541132.1 antitoxin Antitoxin
KU664_RS12175 (KU664_12140) 2513530..2513862 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KU664_RS12185 (KU664_12150) 2514185..2515621 + 1437 WP_251347113.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
KU664_RS12195 (KU664_12160) 2515994..2517448 - 1455 WP_008804170.1 AMP nucleosidase -
KU664_RS12200 (KU664_12165) 2517579..2517824 - 246 WP_008804171.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T207841 WP_008804165.1 NZ_CP077805:2513530-2513862 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T207841 NZ_CP103521:4346324-4346581 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT207841 WP_012541132.1 NZ_CP077805:2513272-2513529 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT207841 NZ_CP103521:4346593-4347138 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References