Detailed information of TA system TA020784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1581699..1582225
• Replicon: chromosome
• Accession: NC_004337
• Organism: Shigella flexneri 2a str. 301

Toxin (Protein)

• Gene name: relE
• Uniprot ID: P0C078
• Locus tag: SF1548
• Protein ID: NP_707430.1
• Coordinates: 1581699..1581986 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: P0C080
• Locus tag: SF1549
• Protein ID: NP_707431.1
• Coordinates: 1581986..1582225 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SF1543	1576757..1577092	+	336	NP_707425.1	anti-adapter protein IraM	-
SF1544	1578402..1579223	-	822	NP_707426.1	antitermination protein	-
SF1545	1579238..1579594	-	357	NP_707427.1	endodeoxyribonuclease RUS	-
SF1546	1579607..1580656	-	1050	NP_707428.1	bacteriophage protein	-
SF1547	1581003..1581251	-	249	NP_707429.2	hypothetical protein	-
SF1547a	1581472..1581627	-	156	YP_008439251.1	regulatory protein	-
SF1548	1581699..1581986	-	288	NP_707430.1	hypothetical protein	Toxin
SF1549	1581986..1582225	-	240	NP_707431.1	bifunctional antitoxin/transcriptional repressor RelB	Antitoxin
SF1550	1582552..1583265	-	714	NP_707432.1	insertion sequence element IS600 integrase core domain-containing protein	-
SF1551	1583406..1583708	-	303	NP_707433.1	insertion sequence element IS600 transposase	-
SF1552	1583809..1584789	+	981	NP_707434.1	low-affinity transport protein	-
SF1553	1584878..1586338	+	1461	NP_707435.1	oxidoreductase	-
SF1554	1586374..1586577	-	204	NP_707436.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1565668..1584789	19121

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T20784 NP_707430.1 NC_004337:c1581986-1581699 [Shigella flexneri 2a str. 301]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T20784 NC_004337:c1581986-1581699 [Shigella flexneri 2a str. 301]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT20784 NP_707431.1 NC_004337:c1582225-1581986 [Shigella flexneri 2a str. 301]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT20784 NC_004337:c1582225-1581986 [Shigella flexneri 2a str. 301]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: P0C078

Antitoxin

• AlphaFold DB: P0C080