Detailed information of TA system TA207832

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 3684211..3684688
• Replicon: chromosome
• Accession: NZ_CP077803
• Organism: Rhodospirillum rubrum strain DSM 467

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: Q2RPF4
• Locus tag: KUL73_RS16470
• Protein ID: WP_011390944.1
• Coordinates: 3684419..3684688 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: Q2RPF5
• Locus tag: KUL73_RS16465
• Protein ID: WP_011390943.1
• Coordinates: 3684211..3684435 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUL73_RS16440 (KUL73_16440)	3679322..3680281	+	960	WP_011390938.1	aspartate carbamoyltransferase catalytic subunit	-
KUL73_RS16445 (KUL73_16445)	3680281..3681579	+	1299	WP_011390939.1	dihydroorotase	-
KUL73_RS16450 (KUL73_16450)	3681572..3682222	+	651	WP_011390940.1	glycerol-3-phosphate 1-O-acyltransferase PlsY	-
KUL73_RS16455 (KUL73_16455)	3682319..3682987	+	669	WP_011390941.1	glutathione S-transferase family protein	-
KUL73_RS16460 (KUL73_16460)	3682990..3684114	+	1125	WP_011390942.1	DNA-processing protein DprA	-
KUL73_RS16465 (KUL73_16465)	3684211..3684435	+	225	WP_011390943.1	TraY domain-containing protein	Antitoxin
KUL73_RS16470 (KUL73_16470)	3684419..3684688	+	270	WP_011390944.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUL73_RS16475 (KUL73_16475)	3684724..3685044	-	321	WP_011390945.1	type II toxin-antitoxin system HicB family antitoxin	-
KUL73_RS16480 (KUL73_16480)	3685099..3685293	-	195	WP_237703881.1	type II toxin-antitoxin system HicA family toxin	-
KUL73_RS16485 (KUL73_16485)	3685642..3686649	+	1008	WP_014626531.1	ABC transporter substrate-binding protein	-
KUL73_RS16490 (KUL73_16490)	3686665..3688389	+	1725	WP_011390948.1	iron ABC transporter permease	-
KUL73_RS16495 (KUL73_16495)	3688377..3689456	+	1080	WP_011390949.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Relaxosome protein TraY (4-48)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10548.30 Da
• Isoelectric Point: 10.5999

>T207832 WP_011390944.1 NZ_CP077803:3684419-3684688 [Rhodospirillum rubrum]
MTWKIEFDPSALRELDKLDPQIAARVLRFLRDRVAVLENPRSLGEALKGPRLGAFWKYRVSDYRIIAHIEDDTLRILVLR
IGNRREVYR

Nucleotide

• Length: 270 bp

>T207832 NZ_CP103521:2410511-2410798 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8437.57 Da
• Isoelectric Point: 4.4678

>AT207832 WP_011390943.1 NZ_CP077803:3684211-3684435 [Rhodospirillum rubrum]
MLAIRLPAELETRLEALARATGRTKTFYVREAITAHLEDLEDFYLADQRLADLRAGATQTVALEDVLKRYDLED

Nucleotide

• Length: 225 bp

>AT207832 NZ_CP103521:2410272-2410511 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: Q2RPF4

Antitoxin

• AlphaFold DB: Q2RPF5