Detailed information of TA system TA207825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-DUF971
• Location: 536733..537318
• Replicon: chromosome
• Accession: NZ_CP077803
• Organism: Rhodospirillum rubrum strain DSM 467

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: Q2RX83
• Locus tag: KUL73_RS02400
• Protein ID: WP_011388216.1
• Coordinates: 537022..537318 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: Q2RX84
• Locus tag: KUL73_RS02395
• Protein ID: WP_011388215.1
• Coordinates: 536733..537020 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUL73_RS02385 (KUL73_02385)	534667..536169	+	1503	WP_011388213.1	leucyl aminopeptidase	-
KUL73_RS02390 (KUL73_02390)	536207..536722	+	516	WP_011388214.1	NUDIX pyrophosphatase	-
KUL73_RS02395 (KUL73_02395)	536733..537020	-	288	WP_011388215.1	putative addiction module antidote protein	Antitoxin
KUL73_RS02400 (KUL73_02400)	537022..537318	-	297	WP_011388216.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUL73_RS02405 (KUL73_02405)	537675..538352	+	678	WP_011388217.1	energy-coupling factor ABC transporter permease	-
KUL73_RS02410 (KUL73_02410)	538672..539379	+	708	WP_081467687.1	PD-(D/E)XK nuclease family protein	-
KUL73_RS02415 (KUL73_02415)	539381..540058	+	678	WP_014625944.1	hypothetical protein	-
KUL73_RS02420 (KUL73_02420)	540180..540806	-	627	WP_011388219.1	NAD(P)-binding domain-containing protein	-
KUL73_RS02425 (KUL73_02425)	540912..541805	+	894	WP_011388220.1	LysR substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Uncharacterized protein HI1420 (3-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11036.68 Da
• Isoelectric Point: 10.6462

>T207825 WP_011388216.1 NZ_CP077803:c537318-537022 [Rhodospirillum rubrum]
MIELKQTETFRKWRTRLKDVRARAIIAARLDRLSQGHTGDSEPVGDGISELRIHYGPGYRLSFQKRGQTIVVLLCGGDKS
TQAKDIQTAKRLAEIGKE

Nucleotide

• Length: 297 bp

>T207825 NZ_CP103521:c824539-824162 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10266.70 Da
• Isoelectric Point: 4.5680

>AT207825 WP_011388215.1 NZ_CP077803:c537020-536733 [Rhodospirillum rubrum]
MTETLTPYDPAEDLVSDEAVAVFMTEAFKTEDAAYIAHALGVVARAKGMTQIAKDTGLSREQLYRSFSENGNPTLKTTLA
VMKSLGIELTVKPQA

Nucleotide

• Length: 288 bp

>AT207825 NZ_CP103521:c824997-824629 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: Q2RX83

Antitoxin

• AlphaFold DB: Q2RX84