Detailed information of TA system TA207819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 154370..155040
• Replicon: plasmid pCDI231-348.3
• Accession: NZ_CP077784
• Organism: Klebsiella pneumoniae strain CDI231

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: KUM55_RS28245
• Protein ID: WP_004213072.1
• Coordinates: 154370..154813 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: KUM55_RS28250
• Protein ID: WP_004213073.1
• Coordinates: 154810..155040 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUM55_RS28210 (KUM55_28215)	149783..150058	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
KUM55_RS28215 (KUM55_28220)	150121..150612	+	492	WP_011251327.1	DM13 domain-containing protein	-
KUM55_RS28220 (KUM55_28225)	150661..151581	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
KUM55_RS28225 (KUM55_28230)	151672..152075	+	404	Protein_169	GAF domain-containing protein	-
KUM55_RS28230 (KUM55_28235)	152594..153226	-	633	Protein_170	mucoid phenotype regulator RmpA2	-
KUM55_RS28235 (KUM55_28240)	153643..153947	+	305	Protein_171	transposase	-
KUM55_RS29505	153970..154221	-	252	WP_186987481.1	hypothetical protein	-
KUM55_RS28245 (KUM55_28250)	154370..154813	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
KUM55_RS28250 (KUM55_28255)	154810..155040	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KUM55_RS28255 (KUM55_28260)	155648..156781	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
KUM55_RS28260 (KUM55_28265)	156797..157090	+	294	WP_004213076.1	hypothetical protein	-
KUM55_RS28265 (KUM55_28270)	157080..157286	-	207	WP_004213077.1	hypothetical protein	-
KUM55_RS28270 (KUM55_28275)	157638..157928	+	291	WP_004213078.1	hypothetical protein	-
KUM55_RS28275 (KUM55_28280)	157918..158817	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / mph(A) / sul1 / qacE / dfrA5 / blaNDM-1 / aph(3')-VI / qnrS1 / mph(E) / msr(E) / armA / sul2	rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..348342	348342

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T207819 WP_004213072.1 NZ_CP077784:c154813-154370 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T207819 NZ_CP103516:196812-197117 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT207819 WP_004213073.1 NZ_CP077784:c155040-154810 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT207819 NZ_CP103516:196592-196810 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620