Detailed information of TA system TA207818

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 36459..37369
• Replicon: plasmid pCDI231-348.3
• Accession: NZ_CP077784
• Organism: Klebsiella pneumoniae strain CDI231

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: KUM55_RS27635
• Protein ID: WP_004026354.1
• Coordinates: 36899..37369 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: KUM55_RS27630
• Protein ID: WP_004181895.1
• Coordinates: 36459..36902 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUM55_RS27615 (KUM55_27620)	33092..34579	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
KUM55_RS27620 (KUM55_27625)	34665..35087	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
KUM55_RS27625 (KUM55_27630)	35087..36358	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
KUM55_RS27630 (KUM55_27635)	36459..36902	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
KUM55_RS27635 (KUM55_27640)	36899..37369	+	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
KUM55_RS27640 (KUM55_27645)	37479..37739	-	261	WP_004026352.1	hypothetical protein	-
KUM55_RS27645 (KUM55_27650)	38461..38619	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
KUM55_RS27650 (KUM55_27655)	39222..40196	+	975	WP_058842863.1	hypothetical protein	-
KUM55_RS27655 (KUM55_27660)	40394..40771	-	378	WP_029884711.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / mph(A) / sul1 / qacE / dfrA5 / blaNDM-1 / aph(3')-VI / qnrS1 / mph(E) / msr(E) / armA / sul2	rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..348342	348342

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T207818 WP_004026354.1 NZ_CP077784:36899-37369 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T207818 NZ_CP103516:c195805-195389 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT207818 WP_004181895.1 NZ_CP077784:36459-36902 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT207818 NZ_CP103516:c196032-195802 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A2J4RLY9