Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/ElaA-DUF1778
Location 81760..82511 Replicon plasmid pCDI231-124.3
Accession NZ_CP077783
Organism Klebsiella pneumoniae strain CDI231

Toxin (Protein)


Gene name ataT Uniprot ID H6U1U8
Locus tag KUM55_RS27140 Protein ID WP_014386536.1
Coordinates 81760..82242 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A071LPN3
Locus tag KUM55_RS27145 Protein ID WP_004902250.1
Coordinates 82233..82511 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUM55_RS27120 (KUM55_27125) 78159..78806 - 648 WP_014386537.1 EcsC family protein -
KUM55_RS27125 (KUM55_27130) 78833..79588 - 756 WP_004902235.1 DUF2971 domain-containing protein -
KUM55_RS27130 (KUM55_27135) 79689..80081 - 393 WP_032442757.1 hypothetical protein -
KUM55_RS27135 (KUM55_27140) 80186..80725 - 540 WP_004902239.1 hypothetical protein -
KUM55_RS27140 (KUM55_27145) 81760..82242 - 483 WP_014386536.1 GNAT family N-acetyltransferase Toxin
KUM55_RS27145 (KUM55_27150) 82233..82511 - 279 WP_004902250.1 DUF1778 domain-containing protein Antitoxin
KUM55_RS27150 (KUM55_27155) 82630..82842 - 213 WP_004902255.1 hypothetical protein -
KUM55_RS27155 (KUM55_27160) 82950..83291 - 342 WP_004902257.1 hypothetical protein -
KUM55_RS27160 (KUM55_27165) 84120..84578 - 459 WP_014386535.1 hypothetical protein -
KUM55_RS27165 (KUM55_27170) 85231..85686 - 456 WP_001166628.1 Hg(II)-responsive transcriptional regulator -
KUM55_RS27170 (KUM55_27175) 85758..86123 + 366 WP_032430780.1 mercuric ion transporter MerT -
KUM55_RS27175 (KUM55_27180) 86139..86420 + 282 WP_000732277.1 mercury resistance system periplasmic binding protein MerP -
KUM55_RS27180 (KUM55_27185) 86448..86855 + 408 WP_000523860.1 organomercurial transporter MerC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid catA1 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 - 1..124281 124281


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-144)

Antitoxin

Vibrio phage ICP1, Orf50

(10-86)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17662.45 Da        Isoelectric Point: 8.9130

>T207817 WP_014386536.1 NZ_CP077783:c82242-81760 [Klebsiella pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Download         Length: 483 bp

>T207817 NZ_CP103516:57404-57553 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10704.23 Da        Isoelectric Point: 4.5271

>AT207817 WP_004902250.1 NZ_CP077783:c82511-82233 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Download         Length: 279 bp

>AT207817 NZ_CP103516:c57360-57299 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MBI1

Antitoxin

Source ID Structure
AlphaFold DB A0A071LPN3

References