Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5267010..5267635 Replicon chromosome
Accession NZ_CP077779
Organism Klebsiella pneumoniae strain CDI231

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KUM55_RS25555 Protein ID WP_002882817.1
Coordinates 5267010..5267393 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KUM55_RS25560 Protein ID WP_004150355.1
Coordinates 5267393..5267635 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUM55_RS25540 (KUM55_25545) 5264376..5265278 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KUM55_RS25545 (KUM55_25550) 5265275..5265910 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KUM55_RS25550 (KUM55_25555) 5265907..5266836 + 930 WP_025368581.1 formate dehydrogenase accessory protein FdhE -
KUM55_RS25555 (KUM55_25560) 5267010..5267393 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KUM55_RS25560 (KUM55_25565) 5267393..5267635 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KUM55_RS25565 (KUM55_25570) 5267840..5268757 + 918 WP_025368582.1 alpha/beta hydrolase -
KUM55_RS25570 (KUM55_25575) 5268772..5269713 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KUM55_RS25575 (KUM55_25580) 5269758..5270195 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KUM55_RS25580 (KUM55_25585) 5270192..5271052 - 861 WP_004146232.1 virulence factor BrkB family protein -
KUM55_RS25585 (KUM55_25590) 5271046..5271645 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T207816 WP_002882817.1 NZ_CP077779:c5267393-5267010 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T207816 NZ_CP103515:c4322918-4322745 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT207816 WP_004150355.1 NZ_CP077779:c5267635-5267393 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT207816 NZ_CP103515:c4323242-4322946 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References