Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 94073..94716 Replicon plasmid pCDI694-140.8
Accession NZ_CP077777
Organism Klebsiella pneumoniae strain CDI694

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag KUM43_RS28170 Protein ID WP_001044770.1
Coordinates 94073..94489 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag KUM43_RS28175 Protein ID WP_001261282.1
Coordinates 94486..94716 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUM43_RS28150 (90176) 90176..90448 - 273 Protein_112 transposase -
KUM43_RS28160 (91430) 91430..92452 - 1023 WP_000361404.1 helicase UvrD -
KUM43_RS28165 (92437) 92437..93999 - 1563 WP_004206609.1 AAA family ATPase -
KUM43_RS28170 (94073) 94073..94489 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
KUM43_RS28175 (94486) 94486..94716 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KUM43_RS28180 (94673) 94673..95134 + 462 WP_014343465.1 hypothetical protein -
KUM43_RS28185 (95295) 95295..96239 + 945 WP_011977810.1 hypothetical protein -
KUM43_RS28190 (96276) 96276..96668 + 393 WP_011977811.1 hypothetical protein -
KUM43_RS28195 (96726) 96726..97247 + 522 WP_013214008.1 hypothetical protein -
KUM43_RS28200 (97293) 97293..97496 + 204 WP_011977813.1 hypothetical protein -
KUM43_RS28205 (97526) 97526..98530 + 1005 WP_011977814.1 hypothetical protein -
KUM43_RS28210 (98714) 98714..99493 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-65 / blaSHV-12 / blaKPC-2 - 1..140828 140828


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T207793 WP_001044770.1 NZ_CP077777:c94489-94073 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T207793 NZ_CP103513:c155393-154950 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT207793 WP_001261282.1 NZ_CP077777:c94716-94486 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT207793 NZ_CP103513:c155620-155390 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References