Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5395811..5396436 Replicon chromosome
Accession NZ_CP077773
Organism Klebsiella pneumoniae strain CDI694

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KUM43_RS26640 Protein ID WP_002882817.1
Coordinates 5395811..5396194 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KUM43_RS26645 Protein ID WP_004150355.1
Coordinates 5396194..5396436 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUM43_RS26625 (KUM43_26620) 5393177..5394079 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KUM43_RS26630 (KUM43_26625) 5394076..5394711 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KUM43_RS26635 (KUM43_26630) 5394708..5395637 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KUM43_RS26640 (KUM43_26635) 5395811..5396194 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KUM43_RS26645 (KUM43_26640) 5396194..5396436 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KUM43_RS26650 (KUM43_26645) 5396641..5397558 + 918 WP_002882812.1 alpha/beta hydrolase -
KUM43_RS26655 (KUM43_26650) 5397572..5398513 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KUM43_RS26660 (KUM43_26655) 5398558..5398995 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KUM43_RS26665 (KUM43_26660) 5398992..5399852 - 861 WP_002882807.1 virulence factor BrkB family protein -
KUM43_RS26670 (KUM43_26665) 5399846..5400445 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T207789 WP_002882817.1 NZ_CP077773:c5396194-5395811 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T207789 NZ_CP103512:c4912476-4912192 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT207789 WP_004150355.1 NZ_CP077773:c5396436-5396194 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT207789 NZ_CP103512:c4912708-4912466 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References