Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4199597..4200216 Replicon chromosome
Accession NZ_CP077773
Organism Klebsiella pneumoniae strain CDI694

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KUM43_RS20910 Protein ID WP_002892050.1
Coordinates 4199998..4200216 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KUM43_RS20905 Protein ID WP_002892066.1
Coordinates 4199597..4199971 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUM43_RS20895 (KUM43_20890) 4194749..4195942 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KUM43_RS20900 (KUM43_20895) 4195965..4199111 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
KUM43_RS20905 (KUM43_20900) 4199597..4199971 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KUM43_RS20910 (KUM43_20905) 4199998..4200216 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KUM43_RS20915 (KUM43_20910) 4200375..4200941 + 567 WP_002892030.1 maltose O-acetyltransferase -
KUM43_RS20920 (KUM43_20915) 4200913..4201053 - 141 WP_004147370.1 hypothetical protein -
KUM43_RS20925 (KUM43_20920) 4201074..4201544 + 471 WP_002892026.1 YlaC family protein -
KUM43_RS20930 (KUM43_20925) 4201519..4202970 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KUM43_RS20935 (KUM43_20930) 4203071..4203769 + 699 WP_002892021.1 GNAT family protein -
KUM43_RS20940 (KUM43_20935) 4203766..4203906 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KUM43_RS20945 (KUM43_20940) 4203906..4204169 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T207785 WP_002892050.1 NZ_CP077773:4199998-4200216 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T207785 NZ_CP103512:2039536-2039638 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT207785 WP_002892066.1 NZ_CP077773:4199597-4199971 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT207785 NZ_CP103512:c2039645-2039500 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References