Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4532276..4533086 Replicon chromosome
Accession NZ_CP077763
Organism Klebsiella pneumoniae strain KP63

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag KQQ01_RS22450 Protein ID WP_002887280.1
Coordinates 4532553..4533086 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KQQ01_RS22445 Protein ID WP_002887278.1
Coordinates 4532276..4532542 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQQ01_RS22425 4528148..4528492 - 345 WP_002887266.1 cation efflux system protein CusF -
KQQ01_RS22430 4528510..4529895 - 1386 WP_002887267.1 efflux transporter outer membrane subunit -
KQQ01_RS22435 4530067..4530750 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
KQQ01_RS22440 4530740..4532173 + 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
KQQ01_RS22445 4532276..4532542 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KQQ01_RS22450 4532553..4533086 + 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
KQQ01_RS22455 4533134..4534255 - 1122 WP_002887282.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T207767 WP_002887280.1 NZ_CP077763:4532553-4533086 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T207767 NZ_CP103508:2123823-2123925 [Escherichia coli O25b:H4-ST131]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT207767 WP_002887278.1 NZ_CP077763:4532276-4532542 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT207767 NZ_CP103508:c2123963-2123695 [Escherichia coli O25b:H4-ST131]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References