Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3948881..3949506 Replicon chromosome
Accession NZ_CP077763
Organism Klebsiella pneumoniae strain KP63

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KQQ01_RS19660 Protein ID WP_002882817.1
Coordinates 3949123..3949506 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KQQ01_RS19655 Protein ID WP_004150355.1
Coordinates 3948881..3949123 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQQ01_RS19630 3944872..3945471 + 600 WP_004151865.1 glucose-1-phosphatase -
KQQ01_RS19635 3945465..3946325 + 861 WP_002882807.1 virulence factor BrkB family protein -
KQQ01_RS19640 3946322..3946759 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KQQ01_RS19645 3946804..3947745 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KQQ01_RS19650 3947759..3948676 - 918 WP_002882812.1 alpha/beta hydrolase -
KQQ01_RS19655 3948881..3949123 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KQQ01_RS19660 3949123..3949506 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KQQ01_RS19665 3949680..3950609 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KQQ01_RS19670 3950606..3951241 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KQQ01_RS19675 3951238..3952140 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T207765 WP_002882817.1 NZ_CP077763:3949123-3949506 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T207765 NZ_CP103508:1852177-1852431 [Escherichia coli O25b:H4-ST131]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT207765 WP_004150355.1 NZ_CP077763:3948881-3949123 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT207765 NZ_CP103508:1851929-1852180 [Escherichia coli O25b:H4-ST131]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References