Detailed information of TA system TA020773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 943122..943681
• Replicon: chromosome
• Accession: NC_004310
• Organism: Brucella suis 1330

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: BR_RS04535
• Protein ID: WP_004688321.1
• Coordinates: 943436..943681 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: BR_RS04530
• Protein ID: WP_002964097.1
• Coordinates: 943122..943436 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BR_RS04485	938812..939015	-	204	WP_004690812.1	hypothetical protein	-
BR_RS04490	939018..939233	-	216	WP_002971459.1	hypothetical protein	-
BR_RS04495	939230..939433	-	204	WP_002964088.1	hypothetical protein	-
BR_RS04500	939483..940202	+	720	WP_006072696.1	hypothetical protein	-
BR_RS04505	940199..940429	+	231	WP_002964091.1	hypothetical protein	-
BR_RS04510	940426..940701	+	276	WP_002964092.1	hypothetical protein	-
BR_RS04515	940724..941311	+	588	WP_004683738.1	hypothetical protein	-
BR_RS04520	941546..942256	+	711	WP_004683739.1	porin family protein	-
BR_RS04525	942604..942774	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
BR_RS04530	943122..943436	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
BR_RS04535	943436..943681	-	246	WP_004688321.1	BrnT family toxin	Toxin
BR_RS04540	943814..944452	+	639	Protein_890	tRNA-guanine transglycosylase	-
BR_RS17290	944522..945280	+	759	Protein_891	IS5 family transposase	-
BR_RS04550	945281..946054	+	774	WP_006132649.1	hypothetical protein	-
BR_RS04555	946189..947835	+	1647	WP_004689673.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	937040..951151	14111
	-	flank	IS/Tn	-	-	944522..944890	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T20773 WP_004688321.1 NC_004310:c943681-943436 [Brucella suis 1330]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T20773 NC_004310:c943681-943436 [Brucella suis 1330]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT20773 WP_002964097.1 NC_004310:c943436-943122 [Brucella suis 1330]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT20773 NC_004310:c943436-943122 [Brucella suis 1330]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA