Detailed information of TA system TA020770

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 651366..652014
• Replicon: chromosome
• Accession: NC_004193
• Organism: Oceanobacillus iheyensis HTE831

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: Q8ESK5
• Locus tag: OB_RS03340
• Protein ID: WP_011065028.1
• Coordinates: 651655..652014 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: OB_RS03335
• Protein ID: WP_011065027.1
• Coordinates: 651366..651650 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OB_RS03315	647086..647862	-	777	WP_011065023.1	rhomboid family intramembrane serine protease	-
OB_RS03320	648007..648366	+	360	WP_011065024.1	holo-ACP synthase	-
OB_RS03325	648427..649956	+	1530	WP_011065025.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-
OB_RS03330	649998..651041	+	1044	WP_011065026.1	outer membrane lipoprotein carrier protein LolA	-
OB_RS03335	651366..651650	+	285	WP_011065027.1	hypothetical protein	Antitoxin
OB_RS03340	651655..652014	+	360	WP_011065028.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OB_RS03345	652243..653112	+	870	WP_011065029.1	RsbT co-antagonist protein RsbRA	-
OB_RS03350	653117..653473	+	357	WP_011065030.1	STAS domain-containing protein	-
OB_RS03355	653476..653877	+	402	WP_011065031.1	anti-sigma regulatory factor	-
OB_RS03360	653946..654959	+	1014	WP_011065032.1	PP2C family protein-serine/threonine phosphatase	-
OB_RS03365	655040..655372	+	333	WP_011065033.1	STAS domain-containing protein	-
OB_RS03370	655369..655848	+	480	WP_173338124.1	anti-sigma B factor RsbW	-
OB_RS03375	655820..656611	+	792	WP_011065035.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13264.48 Da
• Isoelectric Point: 9.4865

>T20770 WP_011065028.1 NC_004193:651655-652014 [Oceanobacillus iheyensis HTE831]
MIVQRGEVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFDRNSVILLEQI
RTLDKQRLTDKITKLDKEMMIKINQALEISLGLKDVYGG

Nucleotide

• Length: 360 bp

>T20770 NC_004193:651655-652014 [Oceanobacillus iheyensis HTE831]
TTGATCGTTCAAAGAGGCGAGGTATATTTCGCTGACTTATCCCCTGTTGTTGGATCGGAGCAAGGTGGGGTAAGGCCTGT
CCTAATTCTCCAAAACGACATCGGTAACCGTTTCAGCCCTACTGTTATTGTCGCTGCGATAACAGCACAAATTCAAAAGG
CAAAATTACCAACCCATGTGGAGATTGATGCAAAGCGATATGGATTTGATCGGAATTCCGTAATTCTTTTGGAACAAATC
AGGACGTTGGATAAACAGCGTTTAACGGATAAAATTACGAAATTAGATAAGGAAATGATGATTAAAATTAATCAGGCATT
AGAAATAAGCCTCGGCTTAAAAGATGTATATGGTGGATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10927.31 Da
• Isoelectric Point: 4.3182

>AT20770 WP_011065027.1 NC_004193:651366-651650 [Oceanobacillus iheyensis HTE831]
MSESLQEIMVKMPKNLLSEVDGLMKYENSDLSDFICEATQIYLNHKKEEHIQRFHETMQRGYEEMGRINLTIASEAFQAE
EEAENTLERSVIGV

Nucleotide

• Length: 285 bp

>AT20770 NC_004193:651366-651650 [Oceanobacillus iheyensis HTE831]
TTGTCAGAAAGTTTACAAGAAATCATGGTGAAGATGCCAAAGAACCTACTAAGTGAGGTGGATGGGTTAATGAAATATGA
AAATAGCGATCTAAGCGATTTTATTTGTGAAGCAACGCAAATTTATTTAAACCATAAAAAAGAAGAGCATATTCAGCGCT
TCCACGAAACTATGCAGCGTGGTTATGAAGAAATGGGACGCATTAATTTGACGATTGCTTCTGAGGCCTTTCAAGCTGAA
GAGGAGGCTGAAAACACCCTAGAGCGCTCTGTGATCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A1E5JZQ9