Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2204323..2204837 Replicon chromosome
Accession NZ_CP077738
Organism Staphylococcus aureus strain UMCG578

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag KTE14_RS10870 Protein ID WP_000581792.1
Coordinates 2204323..2204532 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag KTE14_RS10875 Protein ID WP_000138932.1
Coordinates 2204544..2204837 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KTE14_RS10850 (KTE14_10770) 2199834..2201189 - 1356 WP_000611457.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
KTE14_RS10855 (KTE14_10775) 2201204..2202274 - 1071 WP_000159641.1 D-alanine--D-alanine ligase -
KTE14_RS10860 (KTE14_10780) 2202592..2203794 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
KTE14_RS10865 (KTE14_10785) 2204058..2204195 - 138 WP_000828354.1 Lmo0850 family protein -
KTE14_RS10870 (KTE14_10790) 2204323..2204532 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
KTE14_RS10875 (KTE14_10795) 2204544..2204837 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
KTE14_RS10880 (KTE14_10800) 2205003..2206487 + 1485 WP_000571550.1 cardiolipin synthase -
KTE14_RS10885 (KTE14_10805) 2206515..2207162 + 648 WP_001187614.1 HD domain-containing protein -
KTE14_RS10890 2207719..2207781 + 63 Protein_2103 hypothetical protein -
KTE14_RS10895 (KTE14_10815) 2207759..2209074 - 1316 Protein_2104 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T207678 WP_000581792.1 NZ_CP077738:c2204532-2204323 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T207678 NZ_CP103468:4583410-4583784 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT207678 WP_000138932.1 NZ_CP077738:c2204837-2204544 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT207678 NZ_CP103468:4582952-4583320 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References