Detailed information of TA system TA207677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2184586..2185115
• Replicon: chromosome
• Accession: NZ_CP077738
• Organism: Staphylococcus aureus strain UMCG578

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KTE14_RS10780
• Protein ID: WP_000621175.1
• Coordinates: 2184586..2184948 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KTE14_RS10785
• Protein ID: WP_000948331.1
• Coordinates: 2184945..2185115 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KTE14_RS10755 (KTE14_10675)	2180232..2181002	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
KTE14_RS10760 (KTE14_10680)	2180977..2181456	-	480	WP_031863950.1	anti-sigma B factor RsbW	-
KTE14_RS10765 (KTE14_10685)	2181458..2181784	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KTE14_RS10770 (KTE14_10690)	2181852..2183024	-	1173	WP_000195429.1	IS256-like element IS256 family transposase	-
KTE14_RS10775 (KTE14_10695)	2183235..2184236	-	1002	WP_061737662.1	PP2C family protein-serine/threonine phosphatase	-
KTE14_RS10780 (KTE14_10700)	2184586..2184948	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KTE14_RS10785 (KTE14_10705)	2184945..2185115	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KTE14_RS10790 (KTE14_10710)	2185200..2186348	-	1149	WP_001281139.1	alanine racemase	-
KTE14_RS10795 (KTE14_10715)	2186414..2186773	-	360	WP_000581197.1	holo-ACP synthase	-
KTE14_RS10800 (KTE14_10720)	2186777..2187268	-	492	WP_001286980.1	PH domain-containing protein	-
KTE14_RS10805 (KTE14_10725)	2187261..2188838	-	1578	WP_001294650.1	PH domain-containing protein	-
KTE14_RS10810 (KTE14_10730)	2188831..2189310	-	480	WP_001287081.1	hypothetical protein	-
KTE14_RS10815 (KTE14_10735)	2189519..2190079	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2181852..2183024	1172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T207677 WP_000621175.1 NZ_CP077738:c2184948-2184586 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T207677 NZ_CP103468:c4344761-4344474 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGTATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT207677 WP_000948331.1 NZ_CP077738:c2185115-2184945 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT207677 NZ_CP103468:c4344487-4344185 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1