Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yoeB-relB/YoeB-RelB
Location 14548..15052 Replicon plasmid pCM027
Accession NZ_CP077726
Organism Clostridium sp. CM027

Toxin (Protein)


Gene name yoeB Uniprot ID -
Locus tag KTC92_RS18255 Protein ID WP_220287308.1
Coordinates 14795..15052 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag KTC92_RS18250 Protein ID WP_220287307.1
Coordinates 14548..14802 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KTC92_RS18220 (KTC92_18080) 10064..10288 + 225 WP_220287294.1 DUF1659 domain-containing protein -
KTC92_RS18225 (KTC92_18085) 10347..10583 + 237 WP_220287297.1 DUF2922 domain-containing protein -
KTC92_RS18230 (KTC92_18090) 10771..11550 + 780 WP_220287300.1 N-acetylmuramoyl-L-alanine amidase -
KTC92_RS18235 11606..11731 + 126 WP_258280793.1 hypothetical protein -
KTC92_RS18240 (KTC92_18095) 11841..12002 - 162 WP_220287303.1 hypothetical protein -
KTC92_RS18245 (KTC92_18100) 13463..13771 - 309 WP_220287305.1 hypothetical protein -
KTC92_RS18250 (KTC92_18105) 14548..14802 + 255 WP_220287307.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KTC92_RS18255 (KTC92_18110) 14795..15052 + 258 WP_220287308.1 Txe/YoeB family addiction module toxin Toxin
KTC92_RS18260 (KTC92_18115) 15213..15560 - 348 WP_220287309.1 hypothetical protein -
KTC92_RS18265 (KTC92_18120) 15874..17049 - 1176 WP_220287310.1 replication initiation protein -
KTC92_RS18270 (KTC92_18125) 18533..19111 + 579 WP_220287311.1 CPBP family glutamic-type intramembrane protease -
KTC92_RS18275 (KTC92_18130) 19609..19797 - 189 WP_220287312.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..79567 79567


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(1-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-70)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10252.64 Da        Isoelectric Point: 6.9668

>T207673 WP_220287308.1 NZ_CP077726:14795-15052 [Clostridium sp. CM027]
MNKIFSEVSWEHYVYWQSEDKKILRKINELIKDIERNGNECLGKPEALKHELVGYWSRRITDVHRLIYSIDDENIYVASC
KGHYH

Download         Length: 258 bp

>T207673 NZ_CP103468:c3769524-3769222 [Escherichia coli]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCTGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9833.22 Da        Isoelectric Point: 5.1086

>AT207673 WP_220287307.1 NZ_CP077726:14548-14802 [Clostridium sp. CM027]
MIAIGYSNVRQNFKNYCDKATKDFETIIITRERGDNVVMLSESAYNNLMENLYVRSDPTYYNELLKSIDQLKMGKGIKRE
LLDE

Download         Length: 255 bp

>AT207673 NZ_CP103468:c3769186-3768845 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTACTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAATAATCGTAAACTCACCACTG
AAATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References