Detailed information of TA system TA020767

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 760032..760628
• Replicon: chromosome
• Accession: NC_004129
• Organism: Pseudomonas protegens Pf-5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q4KIY8
• Locus tag: PFL_RS03305
• Protein ID: WP_011059021.1
• Coordinates: 760326..760628 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q4KIY9
• Locus tag: PFL_RS03300
• Protein ID: WP_011059020.1
• Coordinates: 760032..760322 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PFL_RS03280	756302..758434	-	2133	WP_011059016.1	TonB-dependent copper receptor	-
PFL_RS03285	758523..758933	-	411	WP_011059017.1	DUF2946 domain-containing protein	-
PFL_RS03290	758989..759468	-	480	WP_026020078.1	copper chaperone PCu(A)C	-
PFL_RS03295	759533..759931	-	399	WP_011059019.1	DUF2946 domain-containing protein	-
PFL_RS03300	760032..760322	-	291	WP_011059020.1	putative addiction module antidote protein	Antitoxin
PFL_RS03305	760326..760628	-	303	WP_011059021.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PFL_RS03310	760812..761540	-	729	WP_011059022.1	cobalt-precorrin-6A reductase	-
PFL_RS03315	761537..762634	-	1098	WP_011059023.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
PFL_RS03320	762728..763942	-	1215	WP_011059024.1	bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase	-
PFL_RS03325	764081..765394	+	1314	WP_041774047.1	precorrin-3B synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-97)

Antitoxin

• Uncharacterized protein HI1420 (3-94)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11499.32 Da
• Isoelectric Point: 10.4481

>T20767 WP_011059021.1 NC_004129:c760628-760326 [Pseudomonas protegens Pf-5]
MFVFEQTPEFSRWLLNLKDPQGKTRVLARIRAAQLGRFGDCEPVGEGVHEIRIHRGPGYRVYFSRHGKMIYLLLIGGDKA
TQKRDIQRARQLACDLKSEG

Nucleotide

• Length: 303 bp

>T20767 NC_004129:c760628-760326 [Pseudomonas protegens Pf-5]
ATGTTCGTTTTCGAGCAGACTCCTGAGTTCAGCCGCTGGCTGCTGAACCTGAAGGACCCGCAAGGGAAAACCCGTGTACT
GGCCAGGATCCGCGCCGCACAGCTGGGCCGTTTTGGCGACTGCGAACCTGTGGGCGAAGGCGTCCACGAGATACGCATCC
ACCGCGGCCCGGGTTACCGGGTGTACTTCTCGCGCCACGGCAAAATGATCTACCTGCTGTTGATCGGCGGCGACAAGGCC
ACCCAGAAACGCGATATCCAACGGGCCAGGCAACTGGCCTGCGACCTCAAGAGCGAGGGCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10423.94 Da
• Isoelectric Point: 5.0939

>AT20767 WP_011059020.1 NC_004129:c760322-760032 [Pseudomonas protegens Pf-5]
MRTPVTRFDAAEYLKTPEDMVAYLDACFEEDTGDGVLIRAALNDIARARGMTQVARDTGLGRESLYKALGSQGNPEFATI
IKVMKALGLKINVSNA

Nucleotide

• Length: 291 bp

>AT20767 NC_004129:c760322-760032 [Pseudomonas protegens Pf-5]
ATGCGCACCCCAGTGACCCGCTTCGACGCGGCGGAGTACCTCAAGACCCCTGAAGACATGGTCGCCTACCTCGACGCCTG
CTTCGAGGAAGACACCGGCGACGGCGTGCTGATCCGCGCCGCACTCAACGACATCGCCCGTGCCCGCGGCATGACCCAGG
TCGCCCGTGACACCGGCCTGGGCCGCGAAAGCCTGTACAAGGCCCTCGGCAGCCAGGGCAACCCGGAGTTCGCGACTATC
ATCAAGGTGATGAAGGCCCTGGGGTTGAAGATCAACGTCAGCAACGCCTGA

Structures

Toxin

• AlphaFold DB: Q4KIY8

Antitoxin

• AlphaFold DB: Q4KIY9