Detailed information of TA system TA207668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1317192..1317798
• Replicon: chromosome
• Accession: NZ_CP077717
• Organism: Saccharolobus shibatae B12

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J5U23_RS07345
• Protein ID: WP_244988840.1
• Coordinates: 1317424..1317798 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J5U23_RS07340
• Protein ID: WP_218260075.1
• Coordinates: 1317192..1317413 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5U23_RS07310 (J5U23_01454)	1312483..1312641	-	159	WP_218267389.1	hypothetical protein	-
J5U23_RS07315 (J5U23_01455)	1312652..1312801	-	150	WP_218261502.1	hypothetical protein	-
J5U23_RS07320 (J5U23_01456)	1313209..1313865	+	657	WP_218267390.1	hypothetical protein	-
J5U23_RS07325 (J5U23_01457)	1313935..1315326	+	1392	WP_218267391.1	AAA family ATPase	-
J5U23_RS07330 (J5U23_01458)	1315411..1315785	+	375	WP_218267392.1	hypothetical protein	-
J5U23_RS07335 (J5U23_01459)	1315787..1317016	-	1230	WP_218267393.1	RNA-guided endonuclease TnpB family protein	-
J5U23_RS07340 (J5U23_01460)	1317192..1317413	+	222	WP_218260075.1	hypothetical protein	Antitoxin
J5U23_RS07345 (J5U23_01461)	1317424..1317798	+	375	WP_244988840.1	type II toxin-antitoxin system VapC family toxin	Toxin
J5U23_RS07350 (J5U23_01462)	1318228..1319136	+	909	WP_218267394.1	hypothetical protein	-
J5U23_RS07355	1319471..1319554	+	84	Protein_1412	transposase	-
J5U23_RS07360 (J5U23_01463)	1319682..1321106	-	1425	WP_218260410.1	phenylacetate--CoA ligase family protein	-
J5U23_RS07365 (J5U23_01464)	1321281..1321823	+	543	WP_218260145.1	adenosylcobinamide amidohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1315787..1317016	1229

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (2-117)

Antitoxin

• Putative antitoxin VapB3-like (1-72)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14278.80 Da
• Isoelectric Point: 9.2317

>T207668 WP_244988840.1 NZ_CP077717:1317424-1317798 [Saccharolobus shibatae B12]
MAKFVTKEKGWEKITEILKDAETSDFALVEVSNVVWKKTVLTRELTEQDAIKAVTIIRYYLPQLLILNKSIDVIERAIEI
SIKEKIPIYDSLYIALAERKGSKLVTGDRKQHEIAKKYVISELI

Nucleotide

• Length: 375 bp

>T207668 NZ_CP103468:c2665217-2665041 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTGAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 9035.28 Da
• Isoelectric Point: 4.9835

>AT207668 WP_218260075.1 NZ_CP077717:1317192-1317413 [Saccharolobus shibatae B12]
MKTVYSLRIDKELREEMEKYNVKWNEEIENFIRRRIEELKKEEIFKKINEILQTMPETKSNSAELVREDRDNS

Nucleotide

• Length: 222 bp

>AT207668 NZ_CP103468:c2664995-2664579 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAACAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTAA