Detailed information of TA system TA207666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/HTH_10(toxin)
• Location: 1877176..1878155
• Replicon: chromosome
• Accession: NZ_CP077715
• Organism: Saccharolobus shibatae strain BEU9

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J5U21_RS10330
• Protein ID: WP_218258149.1
• Coordinates: 1877541..1878155 (+)
• Length: 205 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J5U21_RS10325
• Protein ID: WP_218260273.1
• Coordinates: 1877176..1877523 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5U21_RS10300 (J5U21_02140)	1874179..1874905	-	727	Protein_2016	transposase	-
J5U21_RS10305 (J5U21_02141)	1875245..1875499	-	255	Protein_2017	IS256 family transposase	-
J5U21_RS10310 (J5U21_02142)	1875974..1876405	+	432	WP_218260690.1	universal stress protein	-
J5U21_RS10315 (J5U21_02143)	1876398..1876781	-	384	WP_218258148.1	metal-sulfur cluster assembly factor	-
J5U21_RS10320 (J5U21_02144)	1876829..1877104	+	276	WP_261310602.1	hypothetical protein	-
J5U21_RS10325 (J5U21_02145)	1877176..1877523	+	348	WP_218260273.1	DUF488 domain-containing protein	Antitoxin
J5U21_RS10330 (J5U21_02146)	1877541..1878155	+	615	WP_218258149.1	helix-turn-helix domain-containing protein	Toxin
J5U21_RS10335 (J5U21_02147)	1878200..1878475	-	276	WP_218258150.1	hypothetical protein	-
J5U21_RS10340 (J5U21_02148)	1878624..1879127	+	504	WP_261310046.1	hemerythrin domain-containing protein	-
J5U21_RS10345 (J5U21_02149)	1879232..1879996	+	765	WP_218260691.1	plastocyanin/azurin family copper-binding protein	-
J5U21_RS10350 (J5U21_02150)	1880000..1880347	+	348	WP_218260692.1	hypothetical protein	-
J5U21_RS10355	1880328..1880555	-	228	WP_218260693.1	hypothetical protein	-
J5U21_RS10360 (J5U21_02151)	1880803..1881402	+	600	WP_218258155.1	DUF2249 domain-containing protein	-
J5U21_RS10365 (J5U21_02152)	1881653..1882843	+	1191	WP_218260694.1	amidohydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterioopsin activator-type, HTH domain (145-197)

Antitoxin

• Protein of unknown function DUF488 (3-108)

Sequences

Toxin

Protein

• Length: 205 a.a.
• Molecular weight: 23212.23 Da
• Isoelectric Point: 9.2823

>T207666 WP_218258149.1 NZ_CP077715:1877541-1878155 [Saccharolobus shibatae]
MLKSPFEVTILIEEHPCEVMRIISSTGLKATVDNVKLGDNATDHIVLFEKEVQKDDLIKLKSHSTKVLKLNDSKVWVRTN
GCSVCKILYTSNVVVEKIKVVKERTLLYTLLVPNTIAFKEFLANLTSQGIKFTVLNTSEISSNDLTDRQMEILKLAYKMG
YFDDDRRVTLTELAKQLGISAPTLEEIMRRALRKVVKYYLDKVG

Nucleotide

• Length: 615 bp

>T207666 NZ_CP103468:1136129-1136464 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13720.03 Da
• Isoelectric Point: 10.0168

>AT207666 WP_218260273.1 NZ_CP077715:1877176-1877523 [Saccharolobus shibatae]
MIKIKRVYDPIEKDDGIRILVDRLWPRGIGKNKVDLWLKDIAPSDELRRWYGHDASKWDEFKRKYFEELDANPKMSILLQ
LIRKGENITLLYASKSPYNNAIALKEYLEKKILKQ

Nucleotide

• Length: 348 bp

>AT207666 NZ_CP103468:1135881-1136129 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCTCCTGCAGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGAAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA