Detailed information of TA system TA207621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 63818..64419
• Replicon: plasmid pCFSAN058545_02
• Accession: NZ_CP077708
• Organism: Salmonella enterica subsp. enterica strain CFSAN058545

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: DAX52_RS23805
• Protein ID: WP_001216034.1
• Coordinates: 63818..64198 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: DAX52_RS23810
• Protein ID: WP_001190712.1
• Coordinates: 64198..64419 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DAX52_RS23780 (DAX52_023775)	59258..60742	-	1485	WP_000124159.1	hypothetical protein	-
DAX52_RS23785 (DAX52_023780)	60742..61935	-	1194	WP_108444138.1	terminase	-
DAX52_RS23790 (DAX52_023785)	62022..62474	-	453	WP_001312282.1	late promoter-activating protein (Gp10)	-
DAX52_RS23795 (DAX52_023790)	62563..63606	-	1044	WP_000648827.1	DUF968 domain-containing protein	-
DAX52_RS23800 (DAX52_023795)	63634..63813	-	180	WP_108444139.1	PdcA protein	-
DAX52_RS23805 (DAX52_023800)	63818..64198	-	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
DAX52_RS23810 (DAX52_023805)	64198..64419	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DAX52_RS23815 (DAX52_023810)	64492..64881	-	390	WP_000506727.1	S24 family peptidase	-
DAX52_RS23820 (DAX52_023815)	65056..65628	+	573	WP_108444140.1	DNA-binding protein	-
DAX52_RS23825 (DAX52_023820)	65635..65828	-	194	Protein_64	DNA polymerase III subunit theta	-
DAX52_RS23830 (DAX52_023825)	66143..66418	-	276	WP_143202209.1	hypothetical protein	-
DAX52_RS23835 (DAX52_023830)	66415..67122	-	708	WP_108444141.1	hypothetical protein	-
DAX52_RS23840 (DAX52_023835)	67418..67723	-	306	WP_059331348.1	hypothetical protein	-
DAX52_RS23845 (DAX52_023840)	67713..68099	-	387	WP_108444143.1	hypothetical protein	-
DAX52_RS23850 (DAX52_023845)	68106..68399	-	294	WP_000269002.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..96045	96045

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T207621 WP_001216034.1 NZ_CP077708:c64198-63818 [Salmonella enterica subsp. enterica]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T207621 NZ_CP103450:263334-263621 [Erwinia pyrifoliae]
TTGATTTATAAGGTTAAAATCAGGGCAGAAGCCCTGAAAGAATGGCATGGTTTGGACAGTGCCGTTCGGGAGCAATTTAG
TAAAAAACTTAAAAAATTGCGTGAAAATCCGCATGTACCTTCAGCCAGGCTAACGGGAATAGCGGGATGTTACAAAATAA
AACTCAGGTCTTCCGGCTTTCGGCTGGTATATCAGGTTATTGATGAAGAGCTGATTATAGCTGTGGTAGCTGTTGGAAAA
AGAGAGCGCAGCCAGGCATATAAACTTGCAAGCGAAAGGTTGCGGTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT207621 WP_001190712.1 NZ_CP077708:c64419-64198 [Salmonella enterica subsp. enterica]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT207621 NZ_CP103450:263087-263329 [Erwinia pyrifoliae]
ATGGCAAATCTCATTCTTAGTGATACCAGTGCAAGCGTAAGCGAGCTTAAAAAGAATCCGATGGCTACAGTTGATGCCGG
TTGCGGCTTTCCTGTAGCGATTCTCAACCGCAATCAGCCCGCTTTCTACTGCATCCCTGCACCACTCTATGAGCGCATGC
TGGAAGTGATAGACGATCAGGAACTGGTCACGCTGGTTAAAGCGCGTGAAAATCAACCTCTGATAGATCTCGATCTGGAC
TAA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6