Detailed information of TA system TA207450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 6019619..6020208
• Replicon: chromosome
• Accession: NZ_CP077678
• Organism: Pseudomonas chlororaphis strain X8

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: KS461_RS27350
• Protein ID: WP_258255437.1
• Coordinates: 6019909..6020208 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: KS461_RS27345
• Protein ID: WP_028683469.1
• Coordinates: 6019619..6019912 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KS461_RS27325 (KS461_27225)	6015181..6016344	-	1164	WP_258255436.1	acyltransferase	-
KS461_RS27330 (KS461_27230)	6016539..6017729	-	1191	WP_124309654.1	serine hydrolase domain-containing protein	-
KS461_RS27335 (KS461_27235)	6017856..6018743	+	888	WP_009051074.1	LysR family transcriptional regulator	-
KS461_RS27340 (KS461_27240)	6019131..6019523	+	393	WP_124309655.1	hypothetical protein	-
KS461_RS27345 (KS461_27245)	6019619..6019912	-	294	WP_028683469.1	putative addiction module antidote protein	Antitoxin
KS461_RS27350 (KS461_27250)	6019909..6020208	-	300	WP_258255437.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KS461_RS27355 (KS461_27255)	6020379..6020828	+	450	WP_007924098.1	DUF3828 domain-containing protein	-
KS461_RS27360 (KS461_27260)	6020853..6021863	-	1011	WP_172834887.1	NAD(P)-dependent alcohol dehydrogenase	-
KS461_RS27365 (KS461_27265)	6021921..6022220	-	300	WP_124309658.1	helix-turn-helix transcriptional regulator	-
KS461_RS27370 (KS461_27270)	6022284..6023375	-	1092	WP_258255438.1	copper-containing nitrite reductase	-
KS461_RS27375 (KS461_27275)	6023601..6023843	+	243	WP_007924102.1	exodeoxyribonuclease VII small subunit	-
KS461_RS27380 (KS461_27280)	6023840..6024727	+	888	WP_258255439.1	(2E,6E)-farnesyl diphosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Uncharacterized protein HI1420 (11-92)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11261.01 Da
• Isoelectric Point: 10.6086

>T207450 WP_258255437.1 NZ_CP077678:c6020208-6019909 [Pseudomonas chlororaphis]
MIEIKQIATFMEWENRLKNSRARALIAARIFRLANGLAGDVSPVGEDVSELRIHYGPGYRVYFQQLGSQLVILLCGGDKS
RQGRDIELAKQLARRWSRS

Nucleotide

• Length: 300 bp

>T207450 NZ_CP103178:3361220-3361480 [Parabacteroides distasonis]
ATGGGTTCTAAAGAAAAGTTGATCGAGCATTTTTGTAAACTACCTAAAGATTTCACCTACGAGGAAACTTTAAAATTGCT
TTCTTCTTATGGCTATAACGAGCATAATAAAGGTGCAACGTCAGGTTCTCGCGTGAGATTTAAAAATGAACAGACGGGAC
AATATATTGATATTCATAAACCGCATCCGGGTAGTATTATGAAGGAGTGGATGATGAAAGCGATATATCAGCATTTAAAA
AGTAATGGTTTAATTAAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10444.96 Da
• Isoelectric Point: 5.6642

>AT207450 WP_028683469.1 NZ_CP077678:c6019912-6019619 [Pseudomonas chlororaphis]
MNEPLFDYDPATALRDPQAIALFMADAFETGDAGYIAKALGVVARAKGMTELAHETGLSREQLYRSFSDKGNPTLKTLLA
VMRALGLEMTARVHGAS

Nucleotide

• Length: 294 bp

>AT207450 NZ_CP103178:3361492-3361833 [Parabacteroides distasonis]
ATGGACTATTTGGAATACAAAGGTTATAAAGGTTCTGTTGAGTACAGTAAGGCTGATAACTGTCTTTGCGGAAAGGTACT
TGGTATGAGTAAAGATTTAATTCTTTATGAAGGTAATACTATCGATGAACTTCGTGCGGATTTTGAAGCCGGAGTAGAAA
GCTATCTTGCCGGCTGTTTGGCTGATGGCGTGGAACCTCGTAAACCTTATAGCGGCACGTTAAATATCCGTATTTCACCA
GAAATACATAGCCGAATCGCAGCATTGGCGCAAGAGGCGGGAACTACGATTAACGGGTATATAAAACAGGCTTTGGAGAA
TCAGTTGAAGCTTGCCCATTAA