Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 316231..316844 Replicon chromosome
Accession NZ_CP077678
Organism Pseudomonas chlororaphis strain X8

Toxin (Protein)


Gene name hicA Uniprot ID A0A285EYZ4
Locus tag KS461_RS01400 Protein ID WP_009041685.1
Coordinates 316231..316413 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A554PT90
Locus tag KS461_RS01405 Protein ID WP_009046450.1
Coordinates 316443..316844 (+) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KS461_RS01370 (KS461_01370) 311997..312254 - 258 WP_162094233.1 hypothetical protein -
KS461_RS01375 (KS461_01375) 312307..312795 - 489 WP_162094234.1 DoxX family protein -
KS461_RS01380 (KS461_01380) 312869..313765 - 897 WP_162094235.1 SDR family oxidoreductase -
KS461_RS01385 (KS461_01385) 313794..314303 - 510 WP_162094236.1 DUF1097 domain-containing protein -
KS461_RS01390 (KS461_01390) 314337..315077 - 741 WP_162094237.1 SDR family oxidoreductase -
KS461_RS01395 (KS461_01395) 315216..316106 + 891 WP_202339219.1 LysR family transcriptional regulator -
KS461_RS01400 (KS461_01400) 316231..316413 + 183 WP_009041685.1 type II toxin-antitoxin system HicA family toxin Toxin
KS461_RS01405 (KS461_01405) 316443..316844 + 402 WP_009046450.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KS461_RS01410 (KS461_01410) 316944..317990 - 1047 WP_081362983.1 LacI family DNA-binding transcriptional regulator -
KS461_RS01415 (KS461_01415) 318198..320456 + 2259 WP_258256262.1 TonB-dependent receptor -
KS461_RS01420 (KS461_01420) 320503..321828 + 1326 WP_258256266.1 LLM class flavin-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.08 Da        Isoelectric Point: 10.4588

>T207444 WP_009041685.1 NZ_CP077678:316231-316413 [Pseudomonas chlororaphis]
MRSRELIRMIEEDGWYLIAVKGSHHQYKHLHKPGRVTIPHPDADLPRGTIHSILKQAGLK

Download         Length: 183 bp

>T207444 NZ_CP103066:c2219600-2219424 [Bacillus subtilis]
GTGCTTGAGAAAATGGGTATCGTAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGGTAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14596.44 Da        Isoelectric Point: 4.9133

>AT207444 WP_009046450.1 NZ_CP077678:316443-316844 [Pseudomonas chlororaphis]
MKFPVVLHKDADSGYGVIVPDVPGCFSAGHTAAQAFENVKEALSLHYEGLVADGEPLPQVHEVDVHIDNPDYAGGVWGVI
EFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Download         Length: 402 bp

>AT207444 NZ_CP103066:2219383-2219483 [Bacillus subtilis]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTGCGTATACGCTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A285EYZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A554PT90

References