Detailed information of TA system TA207443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 57443..57968
• Replicon: plasmid pmk4
• Accession: NZ_CP077677
• Organism: Klebsiella sp. PL-2018

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: MKleb_RS29790
• Protein ID: WP_217077203.1
• Coordinates: 57663..57968 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A6C0NE25
• Locus tag: MKleb_RS29785
• Protein ID: WP_032934863.1
• Coordinates: 57443..57661 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKleb_RS29765 (MKleb_5887)	53922..54077	+	156	Protein_63	IS3 family transposase	-
MKleb_RS29770 (MKleb_5888)	54103..54597	-	495	Protein_64	NAD(P)H-dependent oxidoreductase	-
MKleb_RS29775 (MKleb_5890)	54678..55892	+	1215	WP_217076995.1	IS91 family transposase	-
MKleb_RS29780	56042..56745	-	704	Protein_66	transcriptional regulator	-
MKleb_RS29785 (MKleb_5896)	57443..57661	+	219	WP_032934863.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
MKleb_RS29790 (MKleb_5897)	57663..57968	+	306	WP_217077203.1	type II toxin-antitoxin system toxin CcdB	Toxin
MKleb_RS29795 (MKleb_5898)	58131..58292	+	162	WP_187326103.1	hypothetical protein	-
MKleb_RS29800	58285..59078	+	794	Protein_70	site-specific integrase	-
MKleb_RS29805 (MKleb_5902)	59579..60335	+	757	Protein_71	replication initiation protein RepE	-
MKleb_RS30190 (MKleb_5905)	61024..61356	+	333	WP_267460486.1	hypothetical protein	-
MKleb_RS30195	61290..62033	+	744	Protein_73	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..63257	63257

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11629.40 Da
• Isoelectric Point: 5.6919

>T207443 WP_217077203.1 NZ_CP077677:57663-57968 [Klebsiella sp. PL-2018]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLISAHLLSDKVSRELYPVVNAGDESYRMMTTDMASVPVSVIGEEM
ADLSHRENDIKNAINLMFWGV

Nucleotide

• Length: 306 bp

>T207443 NZ_CP103066:c2219405-2219154 [Bacillus subtilis]
ATGAACTTCTCCTTTAGTTCCTACCCATATTATAACATGATCAAGCACATTGCAAACATGAAACGATTCTCATTATGGTT
TACCCATATCACATTCATTGGCTTATTCTTAATGTTTCAACTCATCAAAGATTACTTCAGCAGCGAAGGACAGGCGCTAA
TCAACACAATTTTTGTTGTCACATGTATCATTGCCATATTGTTATGGATCATCTATTGTGTATTCCTTAAACTAAGAAAC
AAGTCACACTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8329.17 Da
• Isoelectric Point: 4.4013

>AT207443 WP_032934863.1 NZ_CP077677:57443-57661 [Klebsiella sp. PL-2018]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Nucleotide

• Length: 219 bp

>AT207443 NZ_CP103066:2219383-2219483 [Bacillus subtilis]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTGCGTATACGCTTTTT

Structures

Antitoxin

• AlphaFold DB: A0A6C0NE25