Detailed information of TA system TA207442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 48016..48752
• Replicon: plasmid pmk4
• Accession: NZ_CP077677
• Organism: Klebsiella sp. PL-2018

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: MKleb_RS29730
• Protein ID: WP_154022109.1
• Coordinates: 48016..48498 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: MKleb_RS29735
• Protein ID: WP_003026799.1
• Coordinates: 48486..48752 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKleb_RS29700 (MKleb_5871)	43344..44372	-	1029	WP_217077195.1	radical SAM protein	-
MKleb_RS29705 (MKleb_5872)	44423..45157	-	735	WP_217077196.1	hypothetical protein	-
MKleb_RS29710 (MKleb_5873)	45164..45496	-	333	WP_154022755.1	SdpI family protein	-
MKleb_RS30110	45964..46065	-	102	Protein_53	ISNCY family transposase	-
MKleb_RS29720	46317..46745	+	429	WP_217077198.1	cell envelope integrity TolA C-terminal domain-containing protein	-
MKleb_RS30115 (MKleb_5876)	46748..47875	+	1128	WP_228740836.1	hypothetical protein	-
MKleb_RS29730 (MKleb_5878)	48016..48498	-	483	WP_154022109.1	GNAT family N-acetyltransferase	Toxin
MKleb_RS29735 (MKleb_5879)	48486..48752	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
MKleb_RS29740	49158..49755	+	598	Protein_58	tail fiber domain-containing protein	-
MKleb_RS29745 (MKleb_5882)	49759..50895	+	1137	WP_217077199.1	colicin-like bacteriocin tRNase domain-containing protein	-
MKleb_RS29750 (MKleb_5883)	51010..51213	-	204	Protein_60	IS110 family transposase	-
MKleb_RS29755 (MKleb_5884)	51261..52088	-	828	WP_217077200.1	universal stress protein	-
MKleb_RS29760 (MKleb_5885)	52107..53585	-	1479	WP_217077202.1	SulP family inorganic anion transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..63257	63257

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.92 Da
• Isoelectric Point: 8.7400

>T207442 WP_154022109.1 NZ_CP077677:c48498-48016 [Klebsiella sp. PL-2018]
VGCVTAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIIVHALTEEAKGFYTHHGFKVSQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T207442 NZ_CP103066:2069553-2069645 [Bacillus subtilis]
ATGTCAACATTCCAGGCATTAATGCTAATGCTTGCAATCGGGTCGTTTATAATCGCCCTATTGACGTATATAGAAAAAAT
AGACCTCCCTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT207442 WP_003026799.1 NZ_CP077677:c48752-48486 [Klebsiella sp. PL-2018]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT207442 NZ_CP103066:c2069799-2069626 [Bacillus subtilis]
TTACAATACATTACAGTGCATTTCTTTAATGAGAAATGCATAAAATAAAAAAGACCGAGGTGCTACCAACACCCCGGCTC
TGTACAAAAAGTTGCCCATCAAAGGGCTTGCTCGATGTGGTTCTAGGTAGGCCTATCCCTTCAGGTTCTGAGGCTCAAGG
GAGGTCTATTTTTT

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66