Detailed information of TA system TA207441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 6933..7458
• Replicon: plasmid pmk4
• Accession: NZ_CP077677
• Organism: Klebsiella sp. PL-2018

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: MKleb_RS29510
• Protein ID: WP_217077203.1
• Coordinates: 7153..7458 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A6C0NE25
• Locus tag: MKleb_RS29505
• Protein ID: WP_032934863.1
• Coordinates: 6933..7151 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKleb_RS29485 (MKleb_5813)	3410..3565	+	156	Protein_4	IS3 family transposase	-
MKleb_RS29490 (MKleb_5814)	3591..4086	-	496	Protein_5	NAD(P)H-dependent oxidoreductase	-
MKleb_RS29495 (MKleb_5816)	4167..5381	+	1215	WP_217076995.1	IS91 family transposase	-
MKleb_RS29500	5532..6235	-	704	Protein_7	transcriptional regulator	-
MKleb_RS29505 (MKleb_5822)	6933..7151	+	219	WP_032934863.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
MKleb_RS29510 (MKleb_5823)	7153..7458	+	306	WP_217077203.1	type II toxin-antitoxin system toxin CcdB	Toxin
MKleb_RS29515 (MKleb_5824)	7621..7782	+	162	WP_187326103.1	hypothetical protein	-
MKleb_RS29520 (MKleb_5825)	7775..8569	+	795	WP_217077205.1	site-specific integrase	-
MKleb_RS29525 (MKleb_5827)	9070..9825	+	756	WP_217077206.1	replication initiation protein RepE	-
MKleb_RS29530 (MKleb_5830)	10515..11681	+	1167	WP_217077207.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..63257	63257

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11629.40 Da
• Isoelectric Point: 5.6919

>T207441 WP_217077203.1 NZ_CP077677:7153-7458 [Klebsiella sp. PL-2018]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLISAHLLSDKVSRELYPVVNAGDESYRMMTTDMASVPVSVIGEEM
ADLSHRENDIKNAINLMFWGV

Nucleotide

• Length: 306 bp

>T207441 NZ_CP103066:c1349034-1348288 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8329.17 Da
• Isoelectric Point: 4.4013

>AT207441 WP_032934863.1 NZ_CP077677:6933-7151 [Klebsiella sp. PL-2018]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Nucleotide

• Length: 219 bp

>AT207441 NZ_CP103066:c1348288-1348118 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A6C0NE25