Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5764428..5764975 Replicon chromosome
Accession NZ_CP077673
Organism Klebsiella sp. PL-2018

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag MKleb_RS27205 Protein ID WP_014227299.1
Coordinates 5764667..5764975 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag MKleb_RS27200 Protein ID WP_014227298.1
Coordinates 5764428..5764664 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKleb_RS27185 (MKleb_5324) 5760635..5761801 + 1167 WP_025107588.1 multidrug effflux MFS transporter -
MKleb_RS27190 (MKleb_5325) 5761962..5762864 + 903 WP_009654080.1 N-acetylmuramic acid 6-phosphate etherase -
MKleb_RS27195 (MKleb_5326) 5762889..5764322 + 1434 WP_025107587.1 PTS N-acetylmuramic acid transporter subunit IIBC -
MKleb_RS27200 (MKleb_5327) 5764428..5764664 + 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
MKleb_RS27205 (MKleb_5328) 5764667..5764975 + 309 WP_014227299.1 CcdB family protein Toxin
MKleb_RS27210 (MKleb_5329) 5765126..5766028 + 903 WP_014227300.1 EamA family transporter -
MKleb_RS27215 (MKleb_5330) 5766032..5766985 - 954 WP_025107586.1 Rpn family recombination-promoting nuclease/putative transposase -
MKleb_RS27220 (MKleb_5331) 5767195..5767488 + 294 WP_014227302.1 YicS family protein -
MKleb_RS27225 (MKleb_5332) 5767512..5768453 - 942 WP_025107585.1 LacI family DNA-binding transcriptional regulator -
MKleb_RS27230 (MKleb_5333) 5768748..5769059 + 312 WP_014227304.1 PTS sugar transporter subunit IIB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T207440 WP_014227299.1 NZ_CP077673:5764667-5764975 [Klebsiella sp. PL-2018]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T207440 NZ_CP103066:518617-518967 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT207440 WP_014227298.1 NZ_CP077673:5764428-5764664 [Klebsiella sp. PL-2018]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT207440 NZ_CP103066:518331-518612 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References