Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1384782..1385401 Replicon chromosome
Accession NZ_CP077673
Organism Klebsiella sp. PL-2018

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag MKleb_RS06475 Protein ID WP_004099646.1
Coordinates 1384782..1385000 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0J2I7Z1
Locus tag MKleb_RS06480 Protein ID WP_025107145.1
Coordinates 1385027..1385401 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKleb_RS06445 (MKleb_1245) 1380827..1381090 + 264 WP_004848323.1 type B 50S ribosomal protein L31 -
MKleb_RS06450 (MKleb_1246) 1381090..1381230 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
MKleb_RS06455 (MKleb_1247) 1381227..1381925 - 699 WP_025107148.1 GNAT family protein -
MKleb_RS06460 (MKleb_1248) 1382027..1383481 + 1455 WP_217076747.1 PLP-dependent aminotransferase family protein -
MKleb_RS06465 (MKleb_1249) 1383456..1383926 - 471 WP_004848329.1 YlaC family protein -
MKleb_RS29865 1383947..1384081 + 135 WP_223226764.1 hypothetical protein -
MKleb_RS06470 (MKleb_1250) 1384053..1384619 - 567 WP_025107146.1 maltose O-acetyltransferase -
MKleb_RS06475 (MKleb_1251) 1384782..1385000 - 219 WP_004099646.1 HHA domain-containing protein Toxin
MKleb_RS06480 (MKleb_1252) 1385027..1385401 - 375 WP_025107145.1 Hha toxicity modulator TomB Antitoxin
MKleb_RS06485 (MKleb_1253) 1385883..1389029 - 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit AcrB -
MKleb_RS06490 (MKleb_1254) 1389052..1390245 - 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-115)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T207427 WP_004099646.1 NZ_CP077673:c1385000-1384782 [Klebsiella sp. PL-2018]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T207427 NZ_CP102993:807791-808201 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAAATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14366.15 Da        Isoelectric Point: 4.8989

>AT207427 WP_025107145.1 NZ_CP077673:c1385401-1385027 [Klebsiella sp. PL-2018]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNACRENPASLSC

Download         Length: 375 bp

>AT207427 NZ_CP102993:807544-807810 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0J2I7Z1

References