Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 729628..730204 Replicon chromosome
Accession NZ_CP077673
Organism Klebsiella sp. PL-2018

Toxin (Protein)


Gene name shpA Uniprot ID A0A1D8JNH2
Locus tag MKleb_RS03430 Protein ID WP_042933975.1
Coordinates 729628..729915 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1D8JNF7
Locus tag MKleb_RS03435 Protein ID WP_025108476.1
Coordinates 729902..730204 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKleb_RS03400 (MKleb_0644) 724968..725486 - 519 WP_004098240.1 FidL-like protein -
MKleb_RS03405 (MKleb_0645) 725483..726283 - 801 WP_014227752.1 winged helix-turn-helix domain-containing protein -
MKleb_RS03410 (MKleb_0646) 726429..727013 - 585 WP_004098242.1 TetR/AcrR family transcriptional regulator -
MKleb_RS03415 (MKleb_0647) 727161..727943 - 783 WP_025108473.1 NAD(P)H-dependent oxidoreductase -
MKleb_RS03420 (MKleb_0648) 728031..728939 + 909 WP_025108474.1 LysR family transcriptional regulator -
MKleb_RS03425 (MKleb_0649) 728933..729376 + 444 WP_025108475.1 FosA family fosfomycin resistance glutathione transferase -
MKleb_RS03430 (MKleb_0650) 729628..729915 + 288 WP_042933975.1 BrnT family toxin Toxin
MKleb_RS03435 (MKleb_0651) 729902..730204 + 303 WP_025108476.1 BrnA antitoxin family protein Antitoxin
MKleb_RS03440 (MKleb_0652) 730325..731668 - 1344 WP_025108477.1 APC family permease -
MKleb_RS03445 (MKleb_0653) 731665..732576 - 912 WP_025108478.1 acetamidase/formamidase family protein -
MKleb_RS03450 (MKleb_0654) 733164..734150 + 987 WP_025108460.1 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IS/Tn fosA - 728933..734150 5217


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11239.69 Da        Isoelectric Point: 8.5899

>T207425 WP_042933975.1 NZ_CP077673:729628-729915 [Klebsiella sp. PL-2018]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Download         Length: 288 bp

>T207425 NZ_CP102993:323357-323725 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11563.17 Da        Isoelectric Point: 9.7962

>AT207425 WP_025108476.1 NZ_CP077673:729902-730204 [Klebsiella sp. PL-2018]
MNMVKHKRDNPDTLTAKRADELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp

>AT207425 NZ_CP102993:323139-323360 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8JNH2

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8JNF7

References