Detailed information of TA system TA207424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1220760..1221677
• Replicon: chromosome
• Accession: NZ_CP077672
• Organism: Bacillus velezensis strain SWUJ1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: KTT68_RS06345
• Protein ID: WP_007407256.1
• Coordinates: 1220931..1221677 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: KTT68_RS06340
• Protein ID: WP_003154807.1
• Coordinates: 1220760..1220930 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KTT68_RS06300	1215992..1217614	+	1623	WP_224224207.1	pyocin knob domain-containing protein	-
KTT68_RS06305	1217627..1217998	+	372	WP_032874603.1	XkdW family protein	-
KTT68_RS06310	1218003..1218200	+	198	WP_007610833.1	XkdX family protein	-
KTT68_RS06315	1218257..1219018	+	762	WP_045207175.1	hypothetical protein	-
KTT68_RS06320	1219071..1219334	+	264	WP_003154815.1	hemolysin XhlA family protein	-
KTT68_RS06325	1219348..1219611	+	264	WP_003154813.1	phage holin	-
KTT68_RS06330	1219625..1220503	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
KTT68_RS06335	1220538..1220663	-	126	WP_003154809.1	hypothetical protein	-
KTT68_RS06340	1220760..1220930	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
KTT68_RS06345	1220931..1221677	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
KTT68_RS06350	1221782..1222780	-	999	WP_044802872.1	inorganic phosphate transporter	-
KTT68_RS06355	1222793..1223410	-	618	WP_032874614.1	DUF47 domain-containing protein	-
KTT68_RS06360	1223696..1225012	-	1317	WP_224224208.1	amino acid permease	-
KTT68_RS06365	1225335..1226285	+	951	WP_224224209.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T207424 WP_007407256.1 NZ_CP077672:c1221677-1220931 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T207424 NZ_CP102989:c14266-13916 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT207424 WP_003154807.1 NZ_CP077672:c1220930-1220760 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT207424 NZ_CP102989:c13911-13609 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14