Detailed information of TA system TA207423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 479918..480555
• Replicon: chromosome
• Accession: NZ_CP077672
• Organism: Bacillus velezensis strain SWUJ1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: KTT68_RS02450
• Protein ID: WP_003156187.1
• Coordinates: 480205..480555 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: KTT68_RS02445
• Protein ID: WP_003156188.1
• Coordinates: 479918..480199 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KTT68_RS02425	476283..476882	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
KTT68_RS02430	476975..477340	+	366	WP_007609580.1	holo-ACP synthase	-
KTT68_RS02435	477505..478512	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
KTT68_RS02440	478629..479798	+	1170	WP_038457028.1	alanine racemase	-
KTT68_RS02445	479918..480199	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
KTT68_RS02450	480205..480555	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
KTT68_RS02455	480673..481494	+	822	WP_003156182.1	STAS domain-containing protein	-
KTT68_RS02460	481499..481864	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
KTT68_RS02465	481867..482268	+	402	WP_003156178.1	anti-sigma regulatory factor	-
KTT68_RS02470	482280..483287	+	1008	WP_044802655.1	PP2C family protein-serine/threonine phosphatase	-
KTT68_RS02475	483351..483680	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
KTT68_RS02480	483677..484159	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
KTT68_RS02485	484125..484913	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
KTT68_RS02490	484913..485515	+	603	WP_082999005.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T207423 WP_003156187.1 NZ_CP077672:480205-480555 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T207423 NZ_CP102988:c98285-97974 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT207423 WP_003156188.1 NZ_CP077672:479918-480199 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT207423 NZ_CP102988:c97977-97558 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATTGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCATATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5