Detailed information of TA system TA020738

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2149382..2149911
• Replicon: chromosome
• Accession: NC_003923
• Organism: Staphylococcus aureus subsp. aureus MW2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MW_RS10865
• Protein ID: WP_000621175.1
• Coordinates: 2149382..2149744 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MW_RS10870
• Protein ID: WP_000948331.1
• Coordinates: 2149741..2149911 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW_RS10840 (MW1988)	2146362..2147132	-	771	WP_001041108.1	RNA polymerase sigma factor SigB	-
MW_RS10845 (MW1989)	2147107..2147586	-	480	WP_001190830.1	anti-sigma B factor RsbW	-
MW_RS10850 (MW1990)	2147588..2147914	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MW_RS10855 (MW1991)	2148033..2149034	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MW_RS10865 (MW1992)	2149382..2149744	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MW_RS10870 (MW1993)	2149741..2149911	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MW_RS10875 (MW1994)	2149996..2151144	-	1149	WP_001281154.1	alanine racemase	-
MW_RS10880 (MW1995)	2151210..2151569	-	360	WP_000581197.1	holo-ACP synthase	-
MW_RS10885 (MW1996)	2151573..2152064	-	492	WP_001205912.1	PH domain-containing protein	-
MW_RS10890 (MW1997)	2152051..2153634	-	1584	WP_001294626.1	PH domain-containing protein	-
MW_RS10895 (MW1998)	2153627..2154106	-	480	WP_001287078.1	hypothetical protein	-
MW_RS10900 (MW1999)	2154315..2154875	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T20738 WP_000621175.1 NC_003923:c2149744-2149382 [Staphylococcus aureus subsp. aureus MW2]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T20738 NC_003923:c2149744-2149382 [Staphylococcus aureus subsp. aureus MW2]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT20738 WP_000948331.1 NC_003923:c2149911-2149741 [Staphylococcus aureus subsp. aureus MW2]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT20738 NC_003923:c2149911-2149741 [Staphylococcus aureus subsp. aureus MW2]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1