Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2545473..2546093 Replicon chromosome
Accession NZ_CP077661
Organism Enterobacter cloacae complex sp. ECL112

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag KQQ05_RS12310 Protein ID WP_015571250.1
Coordinates 2545473..2545691 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag KQQ05_RS12315 Protein ID WP_006809850.1
Coordinates 2545719..2546093 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQQ05_RS12280 2541485..2541745 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
KQQ05_RS12285 2541748..2541888 + 141 WP_032608666.1 type B 50S ribosomal protein L36 -
KQQ05_RS12290 2541885..2542595 - 711 WP_022650493.1 GNAT family protein -
KQQ05_RS12295 2542697..2544157 + 1461 WP_032608667.1 PLP-dependent aminotransferase family protein -
KQQ05_RS12300 2544129..2544596 - 468 WP_022650495.1 YlaC family protein -
KQQ05_RS12305 2544713..2545264 - 552 WP_022650496.1 maltose O-acetyltransferase -
KQQ05_RS12310 2545473..2545691 - 219 WP_015571250.1 HHA domain-containing protein Toxin
KQQ05_RS12315 2545719..2546093 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
KQQ05_RS12320 2546604..2549750 - 3147 WP_022650497.1 multidrug efflux RND transporter permease subunit AcrB -
KQQ05_RS12325 2549773..2550966 - 1194 WP_022650498.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T207343 WP_015571250.1 NZ_CP077661:c2545691-2545473 [Enterobacter cloacae complex sp. ECL112]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T207343 NZ_CP102962:c2156180-2155971 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATTTAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT207343 WP_006809850.1 NZ_CP077661:c2546093-2545719 [Enterobacter cloacae complex sp. ECL112]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT207343 NZ_CP102962:c2156485-2156192 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References