Detailed information of TA system TA207342

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 2000729..2001408
• Replicon: chromosome
• Accession: NZ_CP077661
• Organism: Enterobacter cloacae complex sp. ECL112

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: KQQ05_RS09730
• Protein ID: WP_032608412.1
• Coordinates: 2001067..2001408 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: KQQ05_RS09725
• Protein ID: WP_020456353.1
• Coordinates: 2000729..2001046 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQQ05_RS09685	1996018..1996842	+	825	Protein_1869	DUF945 domain-containing protein	-
KQQ05_RS09690	1997051..1997734	+	684	WP_020456346.1	DeoR family transcriptional regulator	-
KQQ05_RS23025	1997787..1997909	+	123	WP_020456347.1	hypothetical protein	-
KQQ05_RS09695	1997973..1998839	+	867	WP_032608409.1	hypothetical protein	-
KQQ05_RS09700	1998961..1999167	+	207	Protein_1873	DUF905 family protein	-
KQQ05_RS09705	1999273..1999731	+	459	WP_032608410.1	antirestriction protein	-
KQQ05_RS09710	1999741..1999959	+	219	WP_020456350.1	hypothetical protein	-
KQQ05_RS09715	2000002..2000481	+	480	WP_020456351.1	DNA repair protein RadC	-
KQQ05_RS09720	2000490..2000711	+	222	WP_032608411.1	DUF987 domain-containing protein	-
KQQ05_RS09725	2000729..2001046	+	318	WP_020456353.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KQQ05_RS09730	2001067..2001408	+	342	WP_032608412.1	TA system toxin CbtA family protein	Toxin
KQQ05_RS09735	2001663..2002670	+	1008	WP_020456355.1	restriction endonuclease	-
KQQ05_RS09740	2002851..2003720	-	870	WP_032608413.1	HNH endonuclease	-
KQQ05_RS09745	2004021..2004494	+	474	WP_072163380.1	SymE family type I addiction module toxin	-
KQQ05_RS09750	2004574..2004945	-	372	WP_032608414.1	hypothetical protein	-
KQQ05_RS09755	2005060..2005308	-	249	WP_032608415.1	bacteriocin immunity protein	-
KQQ05_RS09760	2005367..2005591	-	225	WP_032608417.1	colicin E3-like toxin immunity protein	-
KQQ05_RS09765	2005664..2005915	-	252	WP_032608418.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	fosA	-	1970349..2021805	51456

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12747.73 Da
• Isoelectric Point: 9.0060

>T207342 WP_032608412.1 NZ_CP077661:2001067-2001408 [Enterobacter cloacae complex sp. ECL112]
MKTLPATTPQAVTLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T207342 NZ_CP102962:c2136593-2136231 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11846.39 Da
• Isoelectric Point: 5.5403

>AT207342 WP_020456353.1 NZ_CP077661:2000729-2001046 [Enterobacter cloacae complex sp. ECL112]
MSNTTWGLQRDITPRLGARLVQEGNWLHYLADRASITGKFSEAECLKLDVVFPNFISQLESMLTTGEMNPSHAYCVTLYH
NGFTCEADTLGSYGYVYIAVYPTQR

Nucleotide

• Length: 318 bp

>AT207342 NZ_CP102962:c2136760-2136590 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAACTCAA
CGAATGAATGA