Detailed information of TA system TA207340

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1030542..1031138
• Replicon: chromosome
• Accession: NZ_CP077661
• Organism: Enterobacter cloacae complex sp. ECL112

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A837F9W8
• Locus tag: KQQ05_RS05075
• Protein ID: WP_023315213.1
• Coordinates: 1030542..1030844 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: KQQ05_RS05080
• Protein ID: WP_032610152.1
• Coordinates: 1030851..1031138 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQQ05_RS05055	1026761..1028110	+	1350	WP_032608241.1	lysine-sensitive aspartokinase 3	-
KQQ05_RS05060	1028204..1029148	+	945	WP_032608239.1	ketopantoate/pantoate/pantothenate transporter PanS	-
KQQ05_RS05065	1029199..1029471	+	273	WP_003860346.1	DUF3811 domain-containing protein	-
KQQ05_RS05070	1029472..1030344	-	873	WP_003860347.1	23S rRNA pseudouridine(2604) synthase RluF	-
KQQ05_RS05075	1030542..1030844	+	303	WP_023315213.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KQQ05_RS05080	1030851..1031138	+	288	WP_032610152.1	putative addiction module antidote protein	Antitoxin
KQQ05_RS05085	1031135..1032766	-	1632	WP_032610151.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11427.21 Da
• Isoelectric Point: 10.1042

>T207340 WP_023315213.1 NZ_CP077661:1030542-1030844 [Enterobacter cloacae complex sp. ECL112]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T207340 NZ_CP102962:c810246-810058 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTCGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10072.60 Da
• Isoelectric Point: 5.7807

>AT207340 WP_032610152.1 NZ_CP077661:1030851-1031138 [Enterobacter cloacae complex sp. ECL112]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISLQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT207340 NZ_CP102962:c810535-810275 [Staphylococcus aureus]
ATGAATAACATTTTGTCAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTTATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A837F9W8