Detailed information of TA system TA020734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1950076..1950852
• Replicon: chromosome
• Accession: NC_003923
• Organism: Staphylococcus aureus subsp. aureus MW2

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: MW_RS09620
• Protein ID: WP_000031108.1
• Coordinates: 1950076..1950228 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: MW_RS09625
• Protein ID: WP_001251224.1
• Coordinates: 1950253..1950852 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW_RS09600 (MW1791)	1946212..1947033	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
MW_RS09605 (MW1792)	1947494..1948879	-	1386	WP_000116229.1	class II fumarate hydratase	-
MW_RS09610 (MW1793)	1949075..1949470	-	396	WP_000901023.1	hypothetical protein	-
MW_RS09620 (MW1794)	1950076..1950228	-	153	WP_000031108.1	hypothetical protein	Toxin
MW_RS09625 (MW1795)	1950253..1950852	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
MW_RS09630 (MW1796)	1951011..1951481	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
MW_RS09635 (MW1797)	1951486..1952613	-	1128	WP_000379989.1	tRNA epoxyqueuosine(34) reductase QueG	-
MW_RS09640 (MW1798)	1952764..1953486	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
MW_RS09645 (MW1799)	1953479..1954936	-	1458	WP_000649907.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T20734 WP_000031108.1 NC_003923:c1950228-1950076 [Staphylococcus aureus subsp. aureus MW2]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T20734 NC_003923:c1950228-1950076 [Staphylococcus aureus subsp. aureus MW2]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT20734 WP_001251224.1 NC_003923:c1950852-1950253 [Staphylococcus aureus subsp. aureus MW2]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT20734 NC_003923:c1950852-1950253 [Staphylococcus aureus subsp. aureus MW2]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4