Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3743121..3743741 Replicon chromosome
Accession NZ_CP077660
Organism Enterobacter cloacae complex sp. ECL78

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag KQQ04_RS17905 Protein ID WP_015571250.1
Coordinates 3743523..3743741 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag KQQ04_RS17900 Protein ID WP_006809850.1
Coordinates 3743121..3743495 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQQ04_RS17890 3738248..3739441 + 1194 WP_022650498.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KQQ04_RS17895 3739464..3742610 + 3147 WP_022650497.1 multidrug efflux RND transporter permease subunit AcrB -
KQQ04_RS17900 3743121..3743495 + 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
KQQ04_RS17905 3743523..3743741 + 219 WP_015571250.1 HHA domain-containing protein Toxin
KQQ04_RS17910 3743950..3744501 + 552 WP_126517401.1 maltose O-acetyltransferase -
KQQ04_RS17915 3744618..3745085 + 468 WP_022650495.1 YlaC family protein -
KQQ04_RS17920 3745057..3746517 - 1461 WP_022650494.1 PLP-dependent aminotransferase family protein -
KQQ04_RS17925 3746619..3747329 + 711 WP_022650493.1 GNAT family protein -
KQQ04_RS17930 3747326..3747466 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
KQQ04_RS17935 3747469..3747729 - 261 WP_015571255.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T207335 WP_015571250.1 NZ_CP077660:3743523-3743741 [Enterobacter cloacae complex sp. ECL78]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T207335 NZ_CP102961:c1964857-1964705 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT207335 WP_006809850.1 NZ_CP077660:3743121-3743495 [Enterobacter cloacae complex sp. ECL78]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT207335 NZ_CP102961:c1965481-1964882 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAATATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References