Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA(toxin)
Location 1623051..1624834 Replicon chromosome
Accession NZ_CP077660
Organism Enterobacter cloacae complex sp. ECL78

Toxin (Protein)


Gene name hipA Uniprot ID A0A8B5ZXW9
Locus tag KQQ04_RS07630 Protein ID WP_022651516.1
Coordinates 1623485..1624834 (-) Length 450 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A837FFV9
Locus tag KQQ04_RS07625 Protein ID WP_003859317.1
Coordinates 1623051..1623488 (-) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQQ04_RS07605 1618070..1619092 + 1023 WP_003859324.1 HTH-type transcriptional regulator GalS -
KQQ04_RS07610 1619388..1620386 + 999 WP_217315672.1 galactose/glucose ABC transporter substrate-binding protein MglB -
KQQ04_RS07615 1620466..1621986 + 1521 WP_003859320.1 galactose/methyl galactoside ABC transporter ATP-binding protein MglA -
KQQ04_RS07620 1622002..1623012 + 1011 WP_003859318.1 galactose/methyl galactoside ABC transporter permease MglC -
KQQ04_RS07625 1623051..1623488 - 438 WP_003859317.1 helix-turn-helix transcriptional regulator Antitoxin
KQQ04_RS07630 1623485..1624834 - 1350 WP_022651516.1 HipA domain-containing protein Toxin
KQQ04_RS07635 1624969..1625679 - 711 WP_003859314.1 outer membrane permeability protein SanA -
KQQ04_RS07640 1625807..1626691 - 885 WP_022651515.1 cytidine deaminase -
KQQ04_RS07645 1626820..1627515 - 696 WP_003859310.1 CidB/LrgB family autolysis modulator -
KQQ04_RS07650 1627512..1627907 - 396 WP_217315673.1 CidA/LrgA family protein -
KQQ04_RS07655 1628076..1629011 + 936 WP_003859306.1 tRNA dihydrouridine(16) synthase DusC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(166-406)

Antitoxin

Cro/C1-type helix-turn-helix domain

(75-128)


Sequences

Toxin        


Download         Length: 450 a.a.        Molecular weight: 51151.85 Da        Isoelectric Point: 4.7049

>T207324 WP_022651516.1 NZ_CP077660:c1624834-1623485 [Enterobacter cloacae complex sp. ECL78]
METLTVQAYLNETWTDIALIKYPGSEKKDWNTTQLDYLTEYAINFLDYDDFHAVSVNHPVSLFFDDHGQPGWLRFIDDII
PAGASRRYWINALDISELPVGQQNFLLLKFGTMSPVGNLRIKESVPEWNKFASTKTFTVADVIDRAADFLDYAQERGAAA
GGATGAGGEAPKLLLRCSETDAIWIDTWQNEPDNRDRYYLVKYPRGNRTEVDCNILRAEFHYYHELNAMGFSTIPVETMR
LEEGSHYPSLWLPRFDIVRNEQDQLVRLGMESVYSLLQKAPGTLLDHETTLRALINKITSSHMVQQQGYHFDIPGFVTEW
VRRDLLNIIFGNSDNHGRNTAFVKAENQIMLSPVYDFAPMKADPEGIPRTLKWSLSCESGGDYNFNTIAQTLAEWIPPAT
LLDALHETAVQLIDLPERLAARGVPEQIMEMPAMGFRYIPDKLSRWGLL

Download         Length: 1350 bp

>T207324 NZ_CP102959:c2058987-2058625 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16185.64 Da        Isoelectric Point: 10.5154

>AT207324 WP_003859317.1 NZ_CP077660:c1623488-1623051 [Enterobacter cloacae complex sp. ECL78]
MKTPNEAESELLQTLAQVRSVNKKRHHDEAEEPDKPSVVKRQRVTQGLTRISTLDRQAVLHAAIRDILLGKITQGEALKR
LRVEVLGLKQDEYAKLVSVSRKTLSDVENNRGNYSADVINKIFKPFGLQTGLVPVSKSLIASLFS

Download         Length: 438 bp

>AT207324 NZ_CP102959:c2059154-2058984 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5ZXW9

Antitoxin

Source ID Structure
AlphaFold DB A0A837FFV9

References