Detailed information of TA system TA207322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 4319896..4320512
• Replicon: chromosome
• Accession: NZ_CP077659
• Organism: Enterobacter cloacae complex sp. ECL72

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KTH07_RS20860
• Protein ID: WP_015569913.1
• Coordinates: 4320141..4320512 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: KTH07_RS20855
• Protein ID: WP_015569912.1
• Coordinates: 4319896..4320138 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KTH07_RS20825	4314955..4315755	+	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-
KTH07_RS20830	4315874..4316473	+	600	WP_003861947.1	glucose-1-phosphatase	-
KTH07_RS20835	4316467..4317348	+	882	WP_003861949.1	virulence factor BrkB family protein	-
KTH07_RS20840	4317345..4317782	+	438	WP_015569909.1	D-aminoacyl-tRNA deacylase	-
KTH07_RS20845	4317827..4318768	+	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
KTH07_RS20850	4318777..4319697	-	921	WP_015569911.1	alpha/beta hydrolase	-
KTH07_RS20855	4319896..4320138	+	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
KTH07_RS20860	4320141..4320512	+	372	WP_015569913.1	PIN domain-containing protein	Toxin
KTH07_RS20865	4320552..4321481	-	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
KTH07_RS20870	4321478..4322113	-	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
KTH07_RS20875	4322110..4323012	-	903	WP_014072386.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13767.90 Da
• Isoelectric Point: 6.4882

>T207322 WP_015569913.1 NZ_CP077659:4320141-4320512 [Enterobacter cloacae complex sp. ECL72]
MEHMAVFDTNILIDLFNNRIEAADAIEHTASHRAISLITWMEVMVSARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T207322 NZ_CP102959:c840355-840167 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT207322 WP_015569912.1 NZ_CP077659:4319896-4320138 [Enterobacter cloacae complex sp. ECL72]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT207322 NZ_CP102959:c840644-840384 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3