Detailed information of TA system TA207301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 127118..127743
• Replicon: plasmid p1_ST865
• Accession: NZ_CP077650
• Organism: Escherichia coli strain ST865

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KTN07_RS23825
• Protein ID: WP_000911317.1
• Coordinates: 127118..127516 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: KTN07_RS23830
• Protein ID: WP_000450532.1
• Coordinates: 127516..127743 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KTN07_RS23810 (123360)	123360..123869	+	510	WP_194874737.1	conjugal transfer entry exclusion protein TraS	-
KTN07_RS23815 (123904)	123904..124614	+	711	WP_230988746.1	conjugal transfer complement resistance protein TraT	-
KTN07_RS23820 (124866)	124866..127109	+	2244	WP_217609470.1	type IV conjugative transfer system coupling protein TraD	-
KTN07_RS23825 (127118)	127118..127516	-	399	WP_000911317.1	type II toxin-antitoxin system VapC family toxin	Toxin
KTN07_RS23830 (127516)	127516..127743	-	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	faeC / faeD / faeE / faeF / faeH / faeI / faeJ	1..138757	138757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14857.14 Da
• Isoelectric Point: 8.5264

>T207301 WP_000911317.1 NZ_CP077650:c127516-127118 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T207301 NZ_CP102955:c2083707-2083498 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT207301 WP_000450532.1 NZ_CP077650:c127743-127516 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT207301 NZ_CP102955:c2084012-2083719 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5