Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 33993..34518 Replicon plasmid p1_ST865
Accession NZ_CP077650
Organism Escherichia coli strain ST865

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag KTN07_RS23260 Protein ID WP_001159871.1
Coordinates 33993..34298 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6D0XUY0
Locus tag KTN07_RS23265 Protein ID WP_062873111.1
Coordinates 34300..34518 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KTN07_RS23230 (29869) 29869..30081 - 213 Protein_28 IS256 family transposase -
KTN07_RS23235 (30165) 30165..30581 - 417 WP_217609478.1 plasmid partitioning/stability family protein -
KTN07_RS23240 (30574) 30574..31554 - 981 WP_194874823.1 plasmid segregation protein ParM -
KTN07_RS23245 (31967) 31967..32275 - 309 WP_000030204.1 molecular chaperone GroEL -
KTN07_RS23250 (32362) 32362..33006 - 645 WP_001144036.1 ParA family protein -
KTN07_RS23255 (33186) 33186..33992 - 807 WP_194874824.1 site-specific integrase -
KTN07_RS23260 (33993) 33993..34298 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
KTN07_RS23265 (34300) 34300..34518 - 219 WP_062873111.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KTN07_RS23270 (35115) 35115..35372 + 258 WP_001685220.1 hypothetical protein -
KTN07_RS23275 (35372) 35372..35962 + 591 WP_194874825.1 hypothetical protein -
KTN07_RS23280 (36140) 36140..36505 + 366 WP_217609473.1 hypothetical protein -
KTN07_RS23285 (36505) 36505..37692 + 1188 WP_217609479.1 IS91 family transposase -
KTN07_RS23290 (38004) 38004..38465 + 462 WP_136762785.1 hypothetical protein -
KTN07_RS23295 (38828) 38828..39274 + 447 WP_064217566.1 AvrPphF family type III effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - faeC / faeD / faeE / faeF / faeH / faeI / faeJ 1..138757 138757


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T207300 WP_001159871.1 NZ_CP077650:c34298-33993 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T207300 NZ_CP102955:c2064102-2063740 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8385.34 Da        Isoelectric Point: 4.5203

>AT207300 WP_062873111.1 NZ_CP077650:c34518-34300 [Escherichia coli]
MKQRITVTVDSDSYQLLRAYDVNISGLVSTTMQNEARRLRAERWLAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT207300 NZ_CP102955:c2064269-2064099 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A6D0XUY0

References