Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3386930..3387584 Replicon chromosome
Accession NZ_CP077649
Organism Escherichia coli strain ST865

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag KTN07_RS16225 Protein ID WP_000244772.1
Coordinates 3387177..3387584 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KTN07_RS16220 Protein ID WP_000354046.1
Coordinates 3386930..3387196 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KTN07_RS16195 (3382099) 3382099..3382842 + 744 WP_000951964.1 SDR family oxidoreductase -
KTN07_RS16200 (3382899) 3382899..3384332 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
KTN07_RS16205 (3384377) 3384377..3384688 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KTN07_RS16210 (3384852) 3384852..3385511 + 660 WP_000250274.1 hemolysin III family protein -
KTN07_RS16215 (3385707) 3385707..3386687 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KTN07_RS16220 (3386930) 3386930..3387196 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KTN07_RS16225 (3387177) 3387177..3387584 + 408 WP_000244772.1 protein YgfX Toxin
KTN07_RS16230 (3387624) 3387624..3388145 - 522 WP_001055874.1 flavodoxin FldB -
KTN07_RS16235 (3388257) 3388257..3389153 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KTN07_RS16240 (3389178) 3389178..3389888 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KTN07_RS16245 (3389894) 3389894..3391627 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T207288 WP_000244772.1 NZ_CP077649:3387177-3387584 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T207288 NZ_CP102953:c2033138-2032776 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT207288 WP_000354046.1 NZ_CP077649:3386930-3387196 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT207288 NZ_CP102953:c2033305-2033135 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References