Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2049851..2050348 Replicon chromosome
Accession NZ_CP077649
Organism Escherichia coli strain ST865

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KTN07_RS09860 Protein ID WP_001173343.1
Coordinates 2049851..2050024 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KTN07_RS09865 Protein ID WP_000398619.1
Coordinates 2050052..2050348 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KTN07_RS09850 (2046521) 2046521..2047978 + 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -
KTN07_RS09855 (2048215) 2048215..2049732 + 1518 WP_001295087.1 lysine--tRNA ligase -
KTN07_RS09860 (2049851) 2049851..2050024 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KTN07_RS09865 (2050052) 2050052..2050348 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KTN07_RS09870 (2050575) 2050575..2050847 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KTN07_RS09875 (2050859) 2050859..2051089 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
KTN07_RS09880 (2051270) 2051270..2052901 + 1632 WP_001216486.1 sensor histidine kinase -
KTN07_RS09885 (2052898) 2052898..2053617 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T207283 WP_001173343.1 NZ_CP077649:c2050024-2049851 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T207283 NZ_CP102952:c2058158-2057949 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT207283 WP_000398619.1 NZ_CP077649:c2050348-2050052 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT207283 NZ_CP102952:c2058463-2058170 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References