Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 230400..231038 Replicon chromosome
Accession NZ_CP077649
Organism Escherichia coli strain ST865

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag KTN07_RS01095 Protein ID WP_000813794.1
Coordinates 230862..231038 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag KTN07_RS01090 Protein ID WP_001270286.1
Coordinates 230400..230816 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KTN07_RS01070 (225552) 225552..226493 - 942 WP_001251315.1 ABC transporter permease -
KTN07_RS01075 (226494) 226494..227507 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
KTN07_RS01080 (227525) 227525..228670 - 1146 WP_217609364.1 ABC transporter substrate-binding protein -
KTN07_RS01085 (228915) 228915..230321 - 1407 WP_000760615.1 PLP-dependent aminotransferase family protein -
KTN07_RS01090 (230400) 230400..230816 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
KTN07_RS01095 (230862) 230862..231038 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
KTN07_RS01100 (231260) 231260..231490 + 231 WP_000494244.1 YncJ family protein -
KTN07_RS01105 (231582) 231582..233543 - 1962 WP_050867317.1 23S rRNA 5-hydroxycytidine C2501 synthase -
KTN07_RS01110 (233616) 233616..234152 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
KTN07_RS01115 (234244) 234244..235419 + 1176 WP_001236258.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T207278 WP_000813794.1 NZ_CP077649:c231038-230862 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T207278 NZ_CP102951:c3173621-3173325 [Pseudomonas sp. LS1212]
ATGGAGAAAAGAACGCCCCACTGCAAGCTATCCGCCCTCAAAGCCTTGACCGAGGCGGGCAAGGTACGCACCACGCATGC
TGCGCGAGTAGGCGCTAATGAGTTAGGGCTTGAGCTTTCCGACATGCTGGCCGTGGTGATGGCGCTTACGCCAGCGGATT
TCTACAAGAGCATGACCACTCACGCCGATCACACGATCTGGCAAGACGTGTACCGCCCAAGCACGCAGGCGGGCGACGTG
TATCTGAAACTGACGGTCATTGATGACGTGCTGATCGTGTCCTTCAAGGAGCTATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT207278 WP_001270286.1 NZ_CP077649:c230816-230400 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT207278 NZ_CP102951:c3173322-3172921 [Pseudomonas sp. LS1212]
ATGAAATGTCCTGTTTGCGGCGCTGCGGAACTGATCCGCGACACCCGCGACCTGCCCTATACCTACAAGGGTGAAACTAC
TGTCATTGCTGCGGTGACTGGCGATTTCTGCCCGGCCTGCGCCGAGTCTGTCTTGGACGCAGTGGAGTCGGATCGTGTTA
TGCGCGAAATGCGTGCTTTCTCGAAACAGGTCAATGCGGCCATTGTCGATCCTGGCTTTATCACCACCGTGCGCAAGAAG
CTCGCGCTCGACCAGCGAGAAGCAGCCGAGATTTTCGGTGGTGGCGTCAATGCGTTTTCGCGCTATGAGAATGGCAAAAC
CAAGCCGCCACTGGCGCTGGTAAAGCTGCTCAAGGTGCTTGATCGTCACCCTGAGCTGCTAAACGAAGTCAGAACAGCAT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References