Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3387285..3387939 Replicon chromosome
Accession NZ_CP077646
Organism Escherichia coli strain ST866

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag KTN06_RS16240 Protein ID WP_000244772.1
Coordinates 3387532..3387939 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KTN06_RS16235 Protein ID WP_000354046.1
Coordinates 3387285..3387551 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KTN06_RS16210 (3382454) 3382454..3383197 + 744 WP_000951964.1 SDR family oxidoreductase -
KTN06_RS16215 (3383254) 3383254..3384687 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
KTN06_RS16220 (3384732) 3384732..3385043 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KTN06_RS16225 (3385207) 3385207..3385866 + 660 WP_000250274.1 hemolysin III family protein -
KTN06_RS16230 (3386062) 3386062..3387042 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KTN06_RS16235 (3387285) 3387285..3387551 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KTN06_RS16240 (3387532) 3387532..3387939 + 408 WP_000244772.1 protein YgfX Toxin
KTN06_RS16245 (3387979) 3387979..3388500 - 522 WP_001055874.1 flavodoxin FldB -
KTN06_RS16250 (3388612) 3388612..3389508 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KTN06_RS16255 (3389533) 3389533..3390243 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KTN06_RS16260 (3390249) 3390249..3391982 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T207261 WP_000244772.1 NZ_CP077646:3387532-3387939 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T207261 NZ_CP102947:c4062967-4062683 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT207261 WP_000354046.1 NZ_CP077646:3387285-3387551 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT207261 NZ_CP102947:c4063203-4062970 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References