Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2795438..2796024 Replicon chromosome
Accession NZ_CP077646
Organism Escherichia coli strain ST866

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag KTN06_RS13250 Protein ID WP_042014162.1
Coordinates 2795438..2795668 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag KTN06_RS13255 Protein ID WP_000593555.1
Coordinates 2795665..2796024 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KTN06_RS13240 (2791475) 2791475..2794210 + 2736 WP_028132003.1 ribosome-associated ATPase/putative transporter RbbA -
KTN06_RS13245 (2794210) 2794210..2795334 + 1125 WP_045171765.1 ABC transporter permease -
KTN06_RS13250 (2795438) 2795438..2795668 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
KTN06_RS13255 (2795665) 2795665..2796024 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KTN06_RS13260 (2796144) 2796144..2796545 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
KTN06_RS13265 (2796551) 2796551..2797357 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
KTN06_RS13270 (2797354) 2797354..2798118 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
KTN06_RS13275 (2798118) 2798118..2798951 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
KTN06_RS13280 (2798948) 2798948..2799892 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T207258 WP_042014162.1 NZ_CP077646:2795438-2795668 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T207258 NZ_CP102947:c3603155-3602853 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT207258 WP_000593555.1 NZ_CP077646:2795665-2796024 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT207258 NZ_CP102947:c3602817-3602476 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References